ABSTRACT
<p><b>OBJECTIVE</b>To study the absorption kinetics of dehydrocavidine in rats' stomachs and intestines.</p><p><b>METHOD</b>The absorption kinetics was investigated by the in situ perfusion in rats and the concentrations of drug perfusion solutions were determined by HPLC.</p><p><b>RESULT</b>The hourly absorption percentages of dehydrocavidine in stomach, small intestine were 8.88%, 2.08%, respectively. Although the absorption rate constants of dehydrocavidine in duodenum and jejunum are more than that in ileum and colon, there is no significance difference between them. The absorption rate constants kept at the same level when the concentrations of drug perfusion solution are at middle and high level. The increase of the pH of perfusion solution didnt significantly affect the absorption rate constants of the drug.</p><p><b>CONCLUSION</b>Dehydrocavidine was absorbed poorly at stomach and all segments of intestine in rats, but the absorptions in stomach are better than intestine. Dehydrocavidine was absorbed mainly via passive transport mechanism between middle and high concentration levels.</p>
Subject(s)
Animals , Male , Rats , Berberine Alkaloids , Pharmacokinetics , Colon , Metabolism , Duodenum , Metabolism , Hydrogen-Ion Concentration , Ileum , Metabolism , Intestines , Metabolism , Jejunum , Metabolism , Rats, Sprague-Dawley , Stomach , MetabolismABSTRACT
AIM:To study the oral bioavailability of Silymarin-loaded Nanoparticles(SM-NP). METHODS: Beagle dogs were employed as experiment animals,Yiganling Tablet and Silymarin Extraction were used as the reference preparations.Three constituents were isolated on Ultimate~(TM) AQ-C_(18) column with mobile phase methanol:water(50∶50) at 0.8 mL/min flow rate using gradient elution and ESI ionic source and negative ion detection.Silybin plasma concentration in rat was determined by LC-M. RESULTS: The pharmacokinetics of the tested preparation and Yiganling Tablet met with one-compartment model.The relative bioavailability of three components of SMNP were higher than that of Yiganling Tablet. CONCLUSION: The results indicate that SM-NP has better bioavailability than the reference preparation,which confirms that nanoparticles is a good carrier for improving oral bioavailability of poorly-soluble drugs.