ABSTRACT
The fatty acid profiles of several fungi of the order Mucorales (Zygomycetes), including "Backusella lamprospora" (Lendner) Benny and R. K. Benj., "Benjamiella youngii" P.M. Kirk, "Circinella simplex" van Tieghem, "Cunninghamella blakesleeana" Lendner, "Mortierella ramanniana" (Möller) Linnem., "Mucor circineloides" f. "janssenii" (Lendner) Schipper, "Mycotypha microspora"Fenner, "Rhizomucor miehei" (Cooney and R. Emerson) Schipper and "Rhizomucor pusillus"(Lindt) Schipper, and of "Volutella"sp. Fr., from the class Ascomycetes, were qualitatively analysed by gas-liquid chromatography in order to determine the taxonomic value of these chemotaxonomic markers. The fatty acids present in all strains were palmitic (16:0), oleic (18:1), linoleic (18:2) and kappa-linoleic (18:3) acid, with the exception that the later was not found in "Volutella" sp. Chemotaxonomic markers for some species and genera were obtained, including a non-identified fatty acid, FAME8 (minimum and maximum of 16.53 and 16.61 minutes) for "Benjaminiella youngii CCT4121. Thechemotaxonomic marker of the order Mucorales was the fatty acid 18:3ômega6, confirming previous data from literature. The results of the present study that qualitative fatty acid analysis can be an important chemotaxonomic tool for the classification of the fungi assigned to the order Mucorales (Zygomycetes).
Subject(s)
Mucorales/classification , Fatty Acids/analysis , Chromatography, Gas , Chromatography, Liquid , BiomarkersABSTRACT
Using transposon tnphoA, classical bacterial genetic assays, SDS-PAGE and Western blot of superficial proteins, we have studied the expression of the 31A fimbriae of two bovine wild-type septicaemic Escherichia coli strains. The genes responsible for encoding colonization factor 31A were located in both the plasmid (strain BZ2468) and the chromosome (strain BZ43). The results obtained using HeLa cell cultures led us to believe that the BZ43 strain could have another colonization factor besides 31A, since one mutatnt, which did not express fiinbriae, was still able to adhere to HeLa cells.