ABSTRACT
Effects of intraventricular injections of GABA, and a GABA agonist, muscimol and an antagonist, picrotoxin on succinate dehydrogenase (SDH) enzyme activity in plasma and a few hypothalamic nuclei of brain of rats have been investigated using biochemical, histochemical and cytophotometric techniques. Results show that SDH decreased by GABA and muscimol treatment, and increased after picrotoxin injection. From the above findings, it is apparent that GABA, muscimol and picrotoxin influence SDH activity of plasma and hypothalamic nuclei.
Subject(s)
Animals , Injections, Intraventricular , Male , Muscimol/administration & dosage , Picrotoxin/administration & dosage , Rats , Rats, Inbred Strains , Succinate Dehydrogenase/metabolism , gamma-Aminobutyric Acid/administration & dosageABSTRACT
Effect of injection in third ventricle of GABA, the GABA agonist muscimol, and the GABA antagonist picrotoxin on the activities of acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and monoamine oxidase (MAO) in serum and succinic dehydrogenase (SDH) in plasma has been studied. Surprisingly, the AChE, BuChE, MAO and SDH enzymes activity were inhibited by GABA and muscimol, while they were enhanced by picrotoxin.
Subject(s)
Acetylcholinesterase/blood , Animals , Butyrylcholinesterase/blood , Cholinesterases/blood , Injections, Intraventricular , Male , Monoamine Oxidase/blood , Muscimol/administration & dosage , Picrotoxin/administration & dosage , Rats , Succinate Dehydrogenase/blood , gamma-Aminobutyric Acid/administration & dosageABSTRACT
Injections into third ventricle of brain of either 4 m Molar or 8 m Molar of GABA were given to intact and ovariectomized female rats to evaluate the effects of these dosages on body weight, food intake, water intake and blood glucose levels. Statistically significant reduction in food and water intake with 8 m Molar GABA was observed in intact female rats. Very precise alterations seen in ovariectomized female rats suggested that GABA could demonstrate more profound effects on body weight, food intake and water intake. Blood glucose level was elevated in both the groups with 4 m Molar GABA, while inhibition was seen with 8 m Molar GABA. These observations have been correlated with trophic hormonal profiles, which are modulated by third ventricle GABA injection or it might have had some intervention on hypothalamic mechanisms regulating these functions.