ABSTRACT
Background: To restore the lost cells of patients diagnosed with Muscular Degenerative diseases, stem cell therapy has recently proved to be an effective strategy. Chorion is an ethically approved reservoir which contains mesenchymal stem cells with self- renewal properties and multilineage differentiation capacity. Therefore, the aim of this study was to evaluate the myogenic differentiation potential of human chorion-derived mesenchymal stem cells [C-MSCs] for the first time
Materials and methods: Chorion was digested by using 0.3 percent Collagenase type II. Cells were characterized by using flowcytometry and differentiated into osteoblast and adipoblast lineages. To induce myogenic differentiation, C-MSCs were cultured in DMEM/F12 and 2 percent FBS supplemented with 10 micrometer of 5-azacytidine overnight. Afterwards, the medium was replaced with DMEM/F12 supplemented with 10 percent FBS for two weeks. Real-time PCR and immunocytochemistry were used to evaluate the expression of myogenic markers
Results: The expression of CD90, CD73 and CD44 antigen and their ability to differentiation into osteoblast and adipoblast lineages were confirmed. Although the expression of CTNT and MYH6 reduced second week, Real-time PCR results revealed significant upregulation of Desmin, MYH6 and Cardiac TroponinT at the end of the first week [P<0.05]. Slight upregulation of MYOD and GATA-4 transcripts at first and second week were observed. ICC staining approved the expression of Desmin, cTnT and ?-MHC
Conclusion: The results showed that C-MSCs were potent to differentiate into myoblast