ABSTRACT
Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.
O uso do HPLC na identificação e quantificação dos teores de isoflavonas em amostras obtidas de farmácias de manipulação. Atualmente há um grande interesse na pesquisa e produção de alimentos funcionais, como as isoflavonas, que apresentam comprovada ação na prevenção de males, como o câncer e as doenças cardiovasculares. Considerando a importância da padronização dos suplementos de soja, o objetivo do presente estudo foi de identificar e quantificar as isoflavonas em produtos comercializados em diferentes farmácias de manipulação. Amostras de isoflavonas foram adquiridas em seis diferentes farmácias especializadas em manipulação de fitoterápicos. As isoflavonas foram extraídas, identificadas e quantificadas em CLAE. Nenhuma das amostras, das diferentes farmácias de manipulação, apresentou os teores de isoflavonas indicado no rótulo, ou seja, 20 mg. Quanto ao perfil de isoflavonas, os maiores teores encontrados nas amostras foi de daidzeína com até 66,8 ± 0,09 µg, e os menores de genistina. Sendo assim, é necessário que sejam adotados métodos de padronização e caracterização das matérias-primas utilizadas nas diferentes farmácias de manipulação. Assegurando os teores de isoflavonas, os quais são imprescindíveis para os efeitos prescritos pelos profissionais da área médica.
Subject(s)
Glycine max , Genistein , IsoflavonesABSTRACT
Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.
Nowadays, there is a great interest in the research and the production of functional foods, such as isoflavones. Which present proven action on the prevention of health problems, such as cancer and cardiovascular diseases. Considering the importance of soybean supplements standardization, this study aimed identifying and quantifying the isoflavones in products sold at different pharmacies. Isoflavones samples were acquired in six different pharmacies specialized in the production of phytotherapic medications. The isoflavones were extracted, quantified and identified in HPLC. None of the samples, from different pharmacies, presented the isoflavones content presented on the label, i.e., 20 mg. In relation to the isoflavones profile, the highest levels found were of daidzein, with up to 66.8 ± 0.09 µg, being the lowest levels the ones of genistein. It is necessary, therefore, the adoption of methods for the standardization and characterization of the raw materials used by different pharmacies. It would assure the isoflavones content, once they are essential for the effects prescribed by the professionals in the medical area.