ABSTRACT
Our previous study revealed that the antioxidant activity of polysaccharide [coded as FGFP] extracted from Grifola frondosa by enzymolysis treatment was significantly superior than that [coded as GFP] extracted by boiling-water. In this study, one purified polysaccharide fractions [coded as FGFP-11] was obtained from FGFP by purified using DEAE-52 column and Sephacryl S-500HR column. Results indicated that FGFP-11 with MW of 59.82 kDa consisted of mannose, glucose and galactose with a molar ratio of 1.00:16.36:5.25. Fourier Transform Infrared Spectroscopy [FTIR spectrum] of FGFP-11 was similar with that of polysaccharide extracted by boiling-water from Grifola frondosa. These indicated the enzymolysis did not destroy the polysaccharide structure. NMR spectrum showed that FGFP-11 possess alpha-[1-6] glycosidic bond and alpha-[1-3] glycosidic bond configuration. The experiment of Congo red also revealed that FGFP-11 had triple helix stereo-configuration. Moreover, the antioxidant activities of FGFP-11 were improved compared with that of GFP, especially in scavenging of hydroxyl radical and diphenyl picryl hydrazinyl [DPPH] radical