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Objective:To investigate the protein expression of GTPBP4 in human hepatocelluar carcinoma (HCC) tissues and the influ-ence of GTPBP4 silencing by siRNA on the proliferation and cell cycle of HCC cell line Hep G2. Methods:Western blot analysis was per-formed to observe the protein expression of GTPBP4 in 24 cases of HCC tissues compared with their adjacent noncancerous liver tis-sues. Lentivirus-mediated RNA interference (RNAi) was used to silence GTPBP4 expression in Hep G2, and the infection efficiency was observed under a fluorescence microscope. The silencing effect was tested by Western blot and real-time PCR. After silencing the GT-PBP4 gene, cell proliferation was detected using CCK-8 assay, and the cell cycle was observed using flow cytometry. Results:(1) GT-PBP4 was overexpressed in 21 cases (87.5%) of HCC tissues (P<0.000 1). (2) After the lentivirus with GFP reporter infected the Hep G2 cells, 90%of the cells showed green fluorescence. LV-GTPBP4-RNAi effectively inhibited the expression of GTPBP4 at both mRNA (70%) and protein (67%) levels. (3) The proliferation ability of the LV-GTPBP4-RNAi group significantly decreased after 96 h (inhibition rate:54.51%). Flow cytometry showed that the LV-GTPBP4-RNAi group significantly increased at the G0/G1 phase, whereas the S phase de-creased and arrested at the G0/G1 phase. Conclusion:GTPBP4 overexpression in HCC tissues was associated with hepatocarcinogenesis and promoted tumor cell proliferation, but the specific molecular mechanisms remain to be investigated.
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BACKGROUND:Stem cel is a kind of pluripotent cels with self-replication ability, which can differentiate into various cels under certain conditions. Furthermore, stem cels are rich in a variety of growth factors, which can induce the generation of vessels and nerves, and improve the blood supply of lower limbs, thereby achieving the treatment and preventions of lower limb ischemia OBJECTIVE:To summarize and compare the recent achievements in the theory and therapeutic efficacy of stem cels from different sources in the treatment of diabetic foot. METHODS:The first and second authors retrieved PubMed, Sciencedirect and Medline databases for relevant articles published from January 2000 to January 2015. The key words were “diabetic foot, pathogenesis, stem cel therapy” in English. Initialy, 186 articles were retrieved, and finaly 44 articles were included in result analysis. RESULTS AND CONCLUSION:Stem cels can be a new choice for the treatment of diabetic foot. After stem cel therapy, corresponding symptoms have been aleviated, including the generation of new blood vessels and the reshaping of the colateral vessels, the improvement of motor nerve conduction velocity and nerve reflex, the improvement of the sense of skin pain and temperature, and pain relief. It is stil unclear whether alogeneic stem cels are safe or not, but autologous stem cels, especialy bone marrow mesenchymal stem cels, can be better able to repair damaged vessels and nerves and restore the microcirculation of blood supply. Currently, we need to do more basic and clinical researches to solve the folowing problems: to confirm the effectiveness and safety of stem cel therapy for diabetic foot; to identify whether there is a difference in the differentiation and secretory activity between stem cels in diabetic patients and ordinary people; to give ful play to the treatment of diabetic foot.
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miR-224 is a small noncoding RNA that usually binds to response element of target mRNA through the seed sequences,leading to the suppression of translation or mRNA degradation. Recent researches indicate that miR-224 not on?ly aberrantly expresses in multiple tumors(hepatocellular carcinoma,gastric carcinoma, diffuse large B-cell lymphoma, prostatecancer,colorectal cancer, etc),playing an important role in tumor suppressor or oncognen,but also has an effect on the chemosensitivity of tumor.In this article,we review recent studies about the mechanism of miR-224 in the tumor develop?ment ,progression and chemosensitivity.
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OBJECTlVE To study the effect of eucaIyptus oiI on fetus skeIetaI deveIopment of retin-oic acid( RA)induced teratogenic rats. METHODS Forty-two pregnant SD rats were randomIy and equaIIy divided into 6 groups:eucaIyptus oiI 100,200 and 300 mg·kg-1+RA groups,soIvent group(peanut oiI+RA)group,RA group and normaI group with normaI diet. Three eucaIyptus oiI and soIvent groups were ig given eucaIyptus oiI in 100,200,and 300 mg·kg-1 and peanut oiI 2 mL at the 7th to 14th day of gestation,respectiveIy,and RA 40 mg·kg-1 at the 10th day of gestation. RA group were ig given RA 40 mg·kg-1 at the 10th day of gestation. AII pregnant rats were sacrificed at the 21th day. The Iength of fetus body and taiI as weII as body mass of the embryos were measured. 1/ 2-1/ 3 fetaI rats from each Iitter were randomIy extracted to observe skeIetaI deveIopment under a stereoscope by staining with aIcian bIue and aIizarin red S. The Iength of Iimbs and bone ossification was measured. RESULTS The fetus body mass,body Iength,taiI Iength of normaI group significantIy surpassed those in the other groups(P﹤0.05,P﹤0.01). The fetus body mass and body Iength of each eucaIyptus oiI group were significantIy better than RA group(P﹤0.05,P﹤0.01). The fetus body mass,body Iength and taiI Iength of soIvent group were not significantIy different from those in the RA group. The fetus taiI Iength in eucaIyptus oiI groups had no significant difference compared with RA group. The Iimb and bone ossification of normaI group were Ionger than in RA group(P﹤0.05,P﹤0.01). The Iimb Iength of the eucaIyptus oiI groups was Ionger than in RA group and soIvent controI group(P﹤0.05,P﹤0.01),but there was no difference between the soIvent controI group and RA group. The Iimb ossification of each eucaIyptus oiI group was aIso Ionger than that in RA group and soIvent controI group(P﹤0.05,P﹤0.01),but there was no difference between the soIvent controI group and RA group. CONCLUSlON EucaIyptus oiI can antagonize the retardation of skeIetaI growth and ossification of fetaI rats caused by RA.
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The effects of Lipoid of Mujingzi〔LP,Fruits of vitex neguando L Var cannabifo- lia (Sieb et Zucc) Hand mazz〕 on Smooth muscles are reported. The results were as follows:① In the isolated perfused mice lung-bronchi, LP caused significant increase of perfusion medium dropping through the lung-trachea, and the effect of LP was dose-dependent; ACh (Acetyl-choline)-induced contraction of the isolated mice trachea smooth muscles could be relaxed by LP. ② When LP was given introperitoneally(100~ 300 mg?kg-1) into guinea pig, the incubationperiod of asthma induced by acetycholine together with histamine was prolonged.The optimum dilated effect of LP on smooth muscles of trachea in mice can be concluded as the results of synergistic effects by 4 compositions from LP (Oleic acid,Linoleic acid, Palmitic acid and Stearic acid).