ABSTRACT
The functional characterisation of thousands of
Subject(s)
Genetic Vectors/genetics , Trypanosoma cruzi/genetics , Chromatography, Affinity , Cloning, Molecular , Expressed Sequence Tags/metabolism , Gene Expression/genetics , PlasmidsABSTRACT
Calpains are calcium-dependent cysteine proteinases found in all living organisms and are involved in diverse cellular processes. Calpain-like proteins have been reported after in silico analysis of the Tritryps genome and are believed to play important roles in cell functions of trypanosomatids. We describe the characterization of a member of this family, which is differentially expressed during the life-cycle of Trypanosoma cruzi.