ABSTRACT
To evaluate the effect of high dose thiamine on postprandial blood glucose [PPBG] and hemoglobin A 1C levels in induced diabetic albino rat model. Experimental/Analytical study. Animal House, Isra University Hyderabad from March to October 2013. Forty adult albino rats were divided into four groups; Group I. Controls receiving normal diet, Group II. Controls receiving thiamine fortified diet, Group III. Diabetics receiving normal diet and Group IV. Diabetics receiving thiamine fortified diet. Diabetes mellitus was induced using Streptozocin. Thiamine was given at a dose of 1.6 g/kg body weight. Venous blood samples were drawn from animal's tail with a small bore cannula before and after 12 weeks of experimentation. The PPBG levels and Glycosylated HbA [HbA1c] were measured. The data was converted into database and analyzed on SPSS version 21.0 by ANOVA and Tukey-Kramer's test. A p-value of = 0.05 was taken statistically significant. The PPBG and HbA1c levels were found statistically significant in groups I vs. Ill [p=0.0001], I vs. IV [p=0.0001], II vs. Ill [p=0.0001], II vs. IV, [p=0.001] and III vs. IV [p=0.024] at 12[th] week of experimentation. The study shows significant reduction in the PPBG and HbA1c levels of rats taking thiamine compared to controls [p=0.001]. Significant differences in HbA1c levels were observed between control groups I and II vs. experimental groups III and IV [p=0.024] [p=0.0001] respectively. A highly significant difference in HbA1c was observed in rats given thiamine fortified diet compared to those not given [p=0.001]. The thiamine improves glucose metabolism in induced diabetic rat models, hence it is concluded that the thiamine may be given along with anti- diabetic drugs to overcome defects of glucose metabolism
ABSTRACT
To investigate the protective effect of Pentoxifylline [PTX] in carbon tetrachloride [CC1[4]] induced liver injury in adult male Wistar rat model. Experimental/Analytical study. Animal House, Isra University Hyderabad from May to December 2012. Forty five adult male Wistar rats were divided into three groups; Group A. controls received 0.9% isotonic saline, Group B. received CC1[4] orally [I.9mg/kg] mixed in olive oil, and Group C. received the PTX+ CC1[4]. Blood samples were collected for liver biochemical assays. The animals were sacrificed, liver tissue, after fixation in 4% formaldehyde, was embedded in paraffin. Tissue sections of 5micro thickness were subjected to haematoxylin and eosin staining and were assessed by light microscopy. The data was analyzed on SPSS 21.0 using one-way ANOVA, Tukey-Cramer and Chi-square tests. A p-value of = 0.05 was taken statistically significant. The liver biochemical and histological findings reveal statistically significant differences among the controls, CC1[4] and PTX+ CC1[4] groups [p=0.0001]. Liver enzymes and histology was deranged significantly in CC1[4] group compared to controls and PTX+ CC1[4] group [p=0.0001]. The CC1[4]+PTX group shows less elevation of liver enzymes and derangement in liver histology when compared to CC1[4] group [p=0.001]. The histological findings of congestion, inflammatory cell infiltrate, vacuolar degeneration and necrosis are found prominent in CC1[4] group. The Pentoxifylline protects against oxidative damages caused by carbon tetrachloride induced liver injury in rat model