ABSTRACT
The effects of high dilutions of Cymbopogon winterianus (citronella) on the growth and germination of Sida rhombifolia are analyzed; 5 homeopathic dilutions (3cH, 6cH, 12cH, 24cH, 30cH) and a control (water) were used, with 5 repetitions. Variables analyzed were the primary growth of the root system, length of the shoot, fresh mass total, germination percentage and germination speed index. All dilutions stimulated the primary growth of the root. Dilutions 3cH, 6cH, 12cH and cH stimulated the growth of the aerial parts and 24cH inhibited it. Dilutions 6cH, 12cH, 24cH and 30cH stimulated a larger production of fresh mass than 3cH. Dilution 12cH resulted in the largest germination percentage while 24cH in the lowest. Dilution 12cH resulted in the highest score in the germination speed index, while 3cH and 24cH in the lowest.
Os efeitos de altas diluições de Cymbopogon winterianus (citronella) no crescimento e germinação de Sida rhombifolia são analisados; 5 diluições homeopáticas (3cH, 6cH, 12cH, 24cH, 30cH) e um controle (água) foram utilizados, com 5 repetições. As variáveis analisadas foram: crescimento primário do sistema da raiz, longitude do broto, massa fresca total, porcentagem de germinação e índice de velocidade de germinação. As diluições 3cH, 6cH, 12cH e 30cH estimularam o crescimento das partes aéreas, que foi inibido pela diluição 24cH. As diluições 6cH, 12cH e 30cH estimularam uma maior produção de massa fresca que a diluição 3cH. A diluição 12cH mostrou a porcentagem máxima de germinação, enquanto 24cH mostrou a mínima. Igualmente, a diluição 12cH resultou no máximo índice de velocidade de germinação, enquanto as diluições 3cH e 24cH, os mínimos.
Subject(s)
High Potencies , Growth , Cymbopogon , Dilution , Germination , Homeopathy , Malvaceae , PlantsABSTRACT
Polyacrylamide gel electrophoresis system (PAGE) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases fromAspidosperma polyneuron. The characterization of alpha- and beta-esterases from young leaves of A. polyneuron by the PAGE system showed fourteen esterase isozymes. The differential staining pattern showed that Est-2 isozyme hydrolyzes beta-naphthyl acetate; Est-6, Est-7 and Est-8 isozymes hydrolyze alpha-naphthyl acetate, and Est-1, Est-3, Est-4, Est-5, Est-9, Est-10, Est-11, Est-12, Est-13, and Est-14 isozymes hydrolyze both alpha- and b-naphthyl acetate. Inhibition pattern of a- and beta-esterases showed that Folidol is a more potent inhibitor that Malathion, while Thiamethoxan (an insecticide with organophosphorus-like action) acts as an Est-4 and Est-6 inhibitor and induces the appearance of Est-5 and Est-7 isozymes as more intensely stained bands. Inhibition tests showed that OPC insecticides inhibit or activate plant esterases. Thus, plant esterases may be used as bioindicators to detect the presence and toxicity of residues of topically applied insecticides in agriculture and may be valuable for monitoring pollutants in the environment