Subject(s)
Female , Humans , Male , Middle Aged , Carcinoma, Renal Cell/genetics , Kidney Neoplasms/genetics , Matrix Metalloproteinase 1/genetics , Promoter Regions, Genetic , Polymorphism, Single Nucleotide/genetics , Case-Control Studies , Carcinoma, Renal Cell/enzymology , Gene Frequency , Genetic Predisposition to Disease , Genotype , Kidney Neoplasms/enzymology , Polymorphism, Restriction Fragment LengthABSTRACT
OBJECTIVE: Investigate the possible association of insertion/deletion (2G/G) polymorphism at nucleotide -1607 of the MMP-1 promoter with the development and progression of renal cancer MATERIALS AND METHODS: In this study, we genotyped 217 individuals, 99 patients with renal cell carcinoma (RCC) and 118 controls without cancer. DNA specimens were extracted from epithelial buccal cells and paraffin-embedded tissue of RCC patients and from epithelial buccal cells and blood cells of healthy controls RESULTS: The difference in frequency of 2G/2G genotype between controls (22.9 percent) and RCC patients (28.6 percent) was not statistically significant (p = 0.461). We also did not find correlation between 2G/2G and histological type of RCC. The comparison of genotype distribution and frequency of 2G allele in different populations showed a strong variability of 2G allele frequency among the different ethnic groups. This fact may influence on the collaboration of this 2G allele in RCC or others diseases CONCLUSION: Our data suggest that the matrix metalloproteinase-1 (MMP-1) promoter polymorphism may not play a significant role in renal cell carcinoma patients in Brazil.