ABSTRACT
Several genome-wide association studies identified a strong association of SLC30A8 with type 2 diabetes in individuals of European ancestry The effect of the association of rs 13266634 with type 2 diabetes or related glycemic traits has not been fully extended to non-European populations, and a comprehensive examination of common variants in the gene has not yet been carned out in our population. The aim of the present study was to investigate the association among the polymoiphisms of SLC30A8, and the risk of T2DM and to determine the presence and frequency of single nucleotide polymorphism [SNP] rs13266634 in SLC30A8 gene in T2D patients resident in Ismailia city. SLC30A8 SNP was genotyped using real time PCR allelic discrimination TaqMan assay. A case control study was conducted in 68 cases of type 2 diabetes [51 women and 17 men] and 29 control subjects [13 women and 16 men] from out-patients diabetic clinic of Suez Canal hospital and age and gender were matched. The SNP rs13266634 was evaluated in SLC30A8 C > T genotype. The genotypes of control subjects were 27[93%, CIT], 1 normal homozygot [3.5% C/C] and 1 mutant homozygous [3.5%, T/T]. In diabetic subjects, there were 58 subjects carriers of heterozygous [C/T. 85%], 6 normal homozygots [C/C, 9%] and 4 mutant homozygous [T/T, 6%]. There was significant difference in fasting blood glucose levels in control subjects compared to diabetic subjects' P<0.05, and between control carriers of[CT] genotype compared to genotype [CT] diabetic subjects' P<0.05, also there was significant associations between polymorphisms and the risk of type 2 diabetes in women or men, as there were significant associations in lipids profile [serum high density lipoprotein between control carrier CT genotype compared to diabetic subjects' carrier CT P<0.005, also we found significant difference in body mass index [BMI] between control and diabetic subjects' P<0.05. In addition, none of the SLC30A8 polymorphisms was significantly associated with the age and sex in the control and diabetic subjects. Also, we found higher non significant difference in triglyceride levels, total cholesterol and LDL cholesterol levels in diabetic subjects carrying the C/T genotype comparing to control subjects carrying the C/T genotype. In summary, the data in this study support substantial associations between the common SLC30A8 polymorphisms in gene and the risk of type 2 diabetes. Our results may provide evidence that SLC30A8 is a susceptible locus for type 2 diabetes in our population, and its variant can influence insulin secretion