ABSTRACT
Background: The normal vaginal flora contains a variety of microorganisms. Vaginal discharge is a common gynaecologic problem especially among sexually active females. Pathological vaginal discharges are usually due to infection or inflammation of vagina and/or cervix
Objective: To find out the frequency of aerobic gram negative rods in high vaginal swabs and to determine their antimicrobial susceptibility profile
Materials and Methods: This study was conducted in Microbiology laboratory, Pathology Department, SZMC/H, Rahim Yar Khan from 1[st] January, 2012 to 30[th] June, 2012. High vaginal swabs were collected from 100 females visiting antenatal clinics and gynae OPD of Sheikh Zayed Medical College/Hospital, Rahim Yar Khan. Samples were cultured on blood and Mac Conkey agar plates. Plates were incubated aerobically at 35-37°C. All positive cultures were identified by gram staining, cultural characteristics and biochemical reactions. The antimicrobial susceptibility testing of all isolates was done by standard disc diffusion method
Results: Majority of culture positive females were between 25 to 30 years of age. Escherichia coli [72%] was the predominant organism, followed by Klebsiella spp [16%], Enterobacter [6%], Proteus spp [2%], Morganella [2%], Pseudomonas spp [2%]. All these isolates showed good sensitivity towards imepenem and cefoperazone-Sulbactam
Conclusion: In this study, E.coli were the predominant organism, followed by Klebsiella spp. Imepenem and cefoperazone-sulbactam were the most effective antimicrobials against all isolates