[Compression of three methods of polymerase chain reaction, culture and rapid urease test in diagnosis of Helicobacter pylori in gastric biopsy specimen]

Helicobactr pylori [H.pylori] infection is related to chronic gastritis, peptic ulcer and duodenal ulcer. Thus, identification and treatment of the infection has a considerable importance. The aim of this study was to compare three methods of polymerase chain reaction [PCR], culture and rapid urease test [RUT] in identification of Helicobactr pylori in gastric biopsy specimens. 263 patients [157 woman and 106 man] who suffering from digestive complaints and referred to the endoscopy department of Hajar Hospital in Sharkord [2007, Iran] were participated in the study. Three gastric biopsy samples were collected from each patient. Samples were examined by standard RUT and culture methods for diagnosis of H.pylori. PCR was used for diagnosis of ureC gene. Out of 263 patients, 36.49%, 9.50%, 13.30%, 9.48% and 5.50% had criteria gastritis, gastric ulcer, duodenal ulcer, esophagitis and gastric cancer, respectively. H.pylori infection was diagnosed in 54.37%, 31.94% and 84.79% of the patients by RUT, culture and PCR method, respectively. Findings of this study indicated that PCR has a greater sensitivity rather than rapid urease test and culture methods for diagnosis of H.pylori

[Evaluation of metronidazole resistance Helicobacter pylori strains isolated from Shahrekord]

Metronidazole is one of the most important antibiotics in Helicobacter pylori [H.pylori] eradication regimens. This study was designed to determine metronidazole resistance H. pylori isolates from patients referred to Hajar hospital, Shahrekord, Iran. In this cross-sectional study, 263 patients who referred to endoscopy department of Hajar hospital, Shahrekord in 2007 were randomly selected. Gastric biopsy samples were cultured on selective Brucella agar containing 10% blood and incubated under microerophilic condition at 37°C between 3 and 7 days. Gram stain, urease, catalase and oxidase tests were used to detect H. Pylori isolates, and PCR [polymerase chain reaction] to determine ureC gene. H.pylori indentified. Standard CLSI [Clinical and Laboratory Standard Institute] was used to evaluate metronidazole resistance. Rapid urease test [RUT], culture and PCR detect H. pylori infection in 54.37%, 84% and 84.79%, respectively. Of isolated strains, 49 [58.33%] metronidazole resistance and 7 [8.33%] semisensitive were recognized. Of 49 metronidazole resistant patients, 32.65%, 20.40%, 12.24%, 6.10%, 4.80% and 24.48% had gastritis, gastric ulcer, duodenal ulcer, esophagitis, gastric cancer and normal endoscopy, respectively. Fisher exact test shows that there are relation between metronidazole resistance and women. Regarding high metronidazole resistance among studied patients, alternative antibiotics with less resistance is recommended in Helicobacter pylori [H.pylori] eradication regimens