ABSTRACT
Aim: Our goal was to identify the putative association of rs4072111 variant in IL-16 gene and HCV susceptibility in an Iranian population
Background: Interleukin 16 [IL-16], a multifunctional cytokine, plays a vital role in modulation of immune system
Methods: In present case control and cross sectional study, IL-16 gene variant in 300 patients with hepatitis C [HCV] infection and 300 healthy individuals were analyzed. To evaluate this possible association, genomic DNA from venous blood was extracted and genotypes of IL-16 rs4072111 variant were determined by polymerase chain reaction- Fragments Length Polymorphism Technique [PCR-RFLP]. Then, rs4072111 C/T genotypes frequency and allelic distribution were evaluated in each group
Results: The results of genotyping showed 82% CC, 17.3% CT, 0.7% TT in the control group and 78% CC, 20% CT and 2% TT in the case group. The distribution of rs4072111 C allele was 90.7% in controls and 88% in case group respectively. However, no correlation between IL-16 rs4072111 C/T variants and susceptibility to chronic HCV infection was found in the present study
Conclusion: We concluded the rs4072111 C/T cannot be considered as a proper biomarker to identify susceptibility to chronic hepatitis C virus infection
ABSTRACT
Background: It has been shown by recent studies that there is a significant association between genetic polymorphisms near the regulatory of IL28B gene and response to treatment in viral diseases such as hepatitis C. However, genetic factors involving in infection progression to chronic diseases have not been determined yet. In this study, the association of these IL28B polymorphisms with susceptibility to chronic hepatitis C virus infection has been analyzed
Materials and methods: In this case- control study, 110 patients infected with chronic hepatitis C and 110 healthy individuals were studied. The fragments covering rs8099917 and rs12979860 were amplified by the polymerase chain reaction [PCR] method and genotyped by restriction fragment length polymorphism digestion [RFLP] method using NmuCI and BstUIendonuleases enzymes
Results: Results showed that rs12979860CC genotype were the most frequent which followed by CT and TT. However, rs8099917TT was the dominant genotype. Allele frequencies were included: Rs8099917 T=72.3%, G=27.7% and rs12979860 C=69.5%, T=30.5% at patients and rs8099917 T=80%, G=20% and rs12979860 C=73.2%, T=26.8% at control group
Conclusion: The Results showed that T allele is more prevalent than G at rs12979860 polymorphism and the frequency of C allele is higher than T at rs8099917 position. However, rs809917TT and rs12979860 CC genotypes are the most common genotypes among patients population with chronic hepatitis C