ABSTRACT
Background and Objective: Salmonella is one of the most important zoonotic pathogens responsible for food-borne infections all over the world. Poultry products are widely acknowledged to be a significant reservoir for Salmonella. This study was done to evaluate the antibiotic resistant of Salmonella enterica producer of beta lactamase spectrum in poultry
Methods: In this descriptive - laboratort study 70 Salmonella enterica serotypes were collected from poultry. All Salmonella isolates were tested to antimicrobial susceptibility testing by the Kirby-Bauer disk diffusion according to Clinical Laboratory Standards Institute [CLSI]. Twenty-nine antibiotics were used in this study. Klebsiella pneumoniae; ATCC 700603 was used as quality control strains. The isolates were determined to be ESBL-producing Salmonella by the conventional double-disk synergy and genotypic method
Results: Among 70 salmonella isolates, the most prevalent serotypes were S.typhimurium and S.enteritidis. All serotypes were susceptible to gentamicin, ciprofloxacin, oflaxacin, imipenem, enrofloxacin. The common resistance was observed to cephalexin [96%], cefazolin [96%] and cephalotin [65%]. Among the 70 Salmonella isolates studied, multi-drug resistance was observed in 59 [84%] isolates. Forty-seven [67%] isolates were found to be ESBL-producing isolates. PCR assay of all isolates showed that 17 isolates [33.3%] carried bala CMY2 gene
Conclusion: This study showed that antibiotic resistance to Salmonella enterica serotypes is due to beta lactamase enzyme in this strain is considerably increased in poultry