ABSTRACT
This is a prospective study carried out in 100 cases with the aim to see various methods available to detect H. pylori and their respective yield. Further to select which of the two methods if combined together give the best/highest yield. Department of Medicine Dow University of Health Sciences [DUHS] and Civil Hospital, Karachi. In this study 100 patients were selected coming to Gastrointestinal Clinic of Medical Unit - 5, Civil Hospital and DUHS, Karachi, for various dyspeptic symptoms and were suspected to be suffering from acid peptic disease. Multiple biopsy specimens [six] were taken for the study:-First two samples were directly inoculated into culture media. The third specimen was immerse directly into the helicourease gel for detection of H.pylori. The three biopsy specimens were preserved in the formalin for histological examination and for special staining to detect H.pylori. Finally l0cc blood was drawn to detect both quantitative and qualitative antibody titer for H.pylori. All the tests for detection of H.pylori were performed on these 100 patients and it was found that Enzyme Linked Immunosorbent Assay [ELISA] 82% [Quantitative], H. Flex 72% [Qualitative], Histology 66%, Helicourease test 52%, Culture 46% and ELISA and Histology combine74% positivity. Serological detection on IgG antibodies to H.pylori is found to be highly reliable for the diagnosis of H.pylori infection, because of its non-invasiveness, easy and quick to perform its reliability had made it to replace the invasive procedure as a primary diagnostic test. It is observed that if ELISA and histology are combined together give the highest yield, as histology is the most confirmative procedure to identify the active invasion of the organism into the substance of tissue