ABSTRACT
Statement of the Problem: Candida species are believed to play an important role in initiation and progression of denture stomatitis. The type of the denture material also influences the adhesion of candida and development of stomatitis
Purpose: The aim of this study was comparing the adherence of candida albicans to the conventional and injection molding acrylic denture base materials
Materials and Method: Twenty injection molding and 20 conventional pressure pack acrylic discs [10×10×2 mm] were prepared according to their manufacturer's instructions. Immediately before the study, samples were placed in sterile water for 3 days to remove residual monomers. The samples were then sterilized using an ultra-violet light unit for 10 minutes. 1×10[8] Cfu/ml suspension of candida albicans ATCC-10231 was prepared from 48 h cultured organism on sabouraud dextrose agar plates incubated at 37[degree sign]C. 100 micro L of this suspension was placed on the surface of each disk. After being incubated at 37[degree sign]C for 1 hour, the samples were washed with normal saline to remove non-adherent cells. Attached cells were counted using the colony count method after shaking at 3000 rmp for 20 seconds. Finally, each group was tested for 108 times and the data were statistically analyzed by t-test
Results: Quantitative analysis revealed that differences in colony count average of candida albicans adherence to conventional acrylic materials [8.3×10[3]] comparing to injection molding acrylic resins [6×10[3]] were statistically significant [p<0.001]
Conclusion: Significant reduction of candida albicans adherence to the injection acrylic resin materials makes them valuable for patients with high risk of denture stomatitis
ABSTRACT
Hbs-Ag positivity rates in dialysis patients correlate with endemicity in the general population. There is a subset of patients with occult HBV infection that defined as the presence of detectable HBV-DNA by PCR in patients who are negative for Hbs-Ag. We decided to detect the occult HBV cases in our hemodialysis patients. With support of charity society of renal patients of Qazvin, a cross sectional study was scheduled. We tested all the 134 hemodialysis patients in Buali Hospital of Qazvin university of medical sciences for viral hepatitis B in 1 year period. All the patients were chronic hemodialysis patients. Then Hbc-Ab and Hbs-Ag were detected by ELISA tests. Duplication is considered to have true test results. After completion of this stage, the aliquoted samples were tested for qualitative HBV-DNA [PCR]. The positive cases for HBV-DNA were tested again in a referral center to ensure of test results. 43% were female and 57% were male cases. We had 5 positive cases for hepatitis B by ELISA tests [3.7%] that 4 of them were Hbc-Ab and HBV-DNA positive also as expected, although 1 of them had negative test result by PCR test unusually. We detected 4 [3%] another positive PCR test results for HBV that had negative tests for Hbs-Ag, called occult HBV cases. 3 [75%] of these occult cases were Hbc-Ab negative and known as seronegative occult cases. No significant association was found with the duration of hemodialysis, age or sex and HCV positivity in these cases. Age of occult HBV cases was significantly more [p=0.02]. As mentioned most of our occult HBV cases were seronegative. It seems that Hbc-Ab detection is not sensitive test in these cases. Segregation of positive HBV cases in hemodialysis units is mandatory and new protocols may be considered to detect the cases for segregation in the future