ABSTRACT
Background: testicular function is modified by maturational gonadostatic control highly susceptible to negative physiologic niche-altering factors like UV-rays
Objective: this study was performed in order to uncover new aspects of Compact Florescent Lamps [CFLs] induced damages on the testicular tissue of rats and evaluating the effect of curcumin on testis of rats after exposure to compact florescent Lamps
Materials and Methods: twenty-four adult male Albino rats were randomly divided into three groups: control group [ethyl oleate 0.2 ml, IP, for 45 days, without CFLs exposure], fluorescent group [ethyl oleate 0.2 ml, IP, daily and treated with 12 hr. CFLs exposure for 45 days] and curcumin group [curcumin 20 micro M, IP along with 12 hr. CFLs exposure for 45 days]. The rats were anesthetized at the end of the experiment. Gonadotropin hormones and prolactin levels were measured; Histopathological and histomorphometrical analysis of the testis was carried out
Results: results of this study showed that CFLs significantly decreased serum levels of follicle stimulating hormone, prolactin, testicular weight, sperm motility, TDI, and SPI. Furthermore, CFLs had no effect on serum levels of luteinizing hormone and sperm count and also, increased abnormal sperm shapes. Our results also showed that curcumin supplementation following CFLs reversed these alterations
Conclusion: these results strongly suggest that CFLs severely impairs testis while curcumin as an antioxidant had protective effects on undesirable effects in testis induced by CFLs
ABSTRACT
Background: Cervical intraepithelial neoplasia [CIN] is a premalignant lesion capable of progressing to cervical cancer. Despite the existing well-defined criteria, the histomorphologic diagnosis is subject to high rates of discordance among pathologists. The aim of this study was to evaluate Ki-67 [MIB- 1], CK17 and p16 [INK4a] [p16] markers by immunohistochemical methods in differentiating CIN from benign cervical lesions
Methods: The present study reviewed and re-classified 77 cervical biopsies, originally diagnosed as 31 non-CIN, and 46 CIN, as 54 non-CIN, and 23 CIN based on at least two similar diagnoses. Immunostaining by Ki67, p16 and CK17 markers was performed on all cases and the results were compared with pervious and consensus diagnosis
Results: The overall agreement between pervious and consensus diagnosis was 67.5% [Kappa=0.39, P<0.001]. The sensitivity and specificity of Ki67 immunostaining were 95.6% and 85.1% respectively, while for p16 the corresponding values were 91.3% and 98.1%. The overall agreement, for both p16 and Ki67, with consensus diagnosis were significant [P<0.001]. The sensitivity and specificity of CK17 negative staining in CIN detection were 39.1% and 40.7% respectively
Conclusion: Ki67 and p16 markers are recommended as complementary tests for differentiating between dysplastic and non-dysplastic lesions. CK17 does not discriminate between immature metaplasia with and without dysplasia