Comparación de distintos métodos para la medición de la respuesta inmune humoral de ratones vacunados con el virus de la fiebre aftosa / [Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus]

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.

Comparación de distintos métodos para la medición de la respuesta inmune humoral de ratones vacunados con el virus de la fiebre aftosa / [Comparison of various methods for the measurement of the humoral immune response in mice vaccinated with aphthous fever virus]

Kinetics of the humoral immune primary response and seven-day secondary response of adult CF1 mice to FMDV O1 Campos adjuvanted in aluminium hydroxide-saponin (AHS) or in oil emulsion (OE) were evaluated by means of ELISA and passive hemagglutination (PH). Analysis of the response to AHS vaccine showed that ELISA measured maximal titres of primary response at 23 days post-vaccination (dpv), and at day 17 of secondary response, while PH detected maximal titres for primary as well as secondary response around day 60 pv. Mice immunized with OE vaccine studied by ELISA presented a plateau of primary response around 40 dpv, while secondary response was maximal around 80 dpv. The same sera tested by PH showed the highest titres for primary response on day 50 pv and secondary response was maximal on day 40 pv. A criterion for the evaluation of vaccination efficiency in the murine model is proposed based on the methods employed in the determination of antibody level.

Expresion de antigenos virales en celulas BHK21 cosechadas a diferentes tiempos post infeccion con aftovirus C. / Expression of viral antigens in BHK21 cells harvested at different times post-infection with aphtovirus C

Se estudiaron los polipeptidos presentes en celulas BHK21 infectadas con aftovirus C2 por inmunodifusion, inmunofluorescencia y electroforesis en gel de poliacrilamida con sus perfiles radiactivos, y su posible relacion con la induccion de anticuerpos protectores en conejos. Los perfiles radiactivos fueron diferentes entre las distintas muestras y las celulas sin infectar Se observo fluorescencia a partir de 60 minutos post infeccion, y alteraciones y cambios morfologicos en las celulas, luego de 120 minutos post infeccion, provocados por efecto citopatico viral.Con los resultados obtenidos se postulo que hasta los 75 minutos post infeccion existiria virus no desadsorbido y penetrante; entre 75 y 120 minutos por infeccion habria progenie viral temprana con posible liberacion prelitica, y luego de 120 minutos post infeccion formacion y liberacion de progenie viral. En un trabajo anterior, demostramos que celulas a los 75, 120 y 210 minutos p.i., inyectados en conejos, indujeron anticuerpos que neutralizaban la accion viral. Correlacionando ese trabajo con este, postulamos que la responsabilidad de la produccion de esos anticuerpos estaria en precursores de polipeptidos estructurales, mas que en estos mismos