Genetic Analyses Of Plasma ApoCII By Two-Dimensional Gel Electrophresis In Two Population Groups Of Panjab.

In order to study genetic variability of apolipoprotein C-II in Ramgarhias and Ramdasias, two-dimensional gel electrophoresis with the combination of isoelectric focusing (IEF) and density gradient SDS-polyacrylamide gel electrophoresis (DG-SDS-PAGE) have been employed. ApoC-II exhibits three common polymorphic alleles CII-1, CII-2 and CII-3 with pooled frequencies of 0.883, 0.110 and 0.007 respectively. The rare allele CII-4 is absent in these two groups. Mean heterozygosity is found to be 0.208+ 0.024 at apoC-II structural locus. The frequency of apoCII-2 in Punjab is observed to be the highest in the world except Ukainers.