ABSTRACT
Skipjack tuna (Katsuwonus pelamis) belongs to the Scombridae family to determine the consistencycharacteristics of tuna fish loins for 28th days at frozen (−18 ± 2°C) storage. The biochemical, microbial, andsensory quality were analyzed by association of official analytical chemists method, plate count, and QualityIndex method (QIM), respectively. Protein, lipid, and moisture content were found to decrease significantly(p < 0.05) over the study period. Nonetheless, the values of ash, total volatile base nitrogen, tri-methyl aminenitrogen, pH, and peroxide increased significantly (p < 0.05). After 28 days of frozen storage, the total bacterialload in tuna fish loins was reduced from 3.8 × 105 to 3.3 × 104 colony forming unit/g. Total coliforms reducedfrom 113 to 5 Most Probable Number (MPN)/g, fecal coliforms declined to undetectable level from 13 MPN/g,and total Salmonella sp. also decreased from 1 MPN/g to unnoticeable, respectively. Organoleptically tunaloins were found in excellent condition up to 14th days although remained acceptable up to 28th days of thestudy period. The present findings should that the skipjack tuna loins can be retained under an acceptable limitfor human consumption at frozen storage conditions up to 28th days.
ABSTRACT
Aims: The main objective of this work was to observe the analgesic activity of Manilkara zapota (leaves) on mice. Study Design: The Present study was designed to observe pharmacological activities of the crude extracts of the plant Manilkara zapota (leaves). The study protocol consisted of Cold extraction at room temperature of the whole plant with distilled methanol. Afterwards, Filtration of the crude Methanolic and Petroleum ether extracts by using the Markin cotton cloth and subsequently through the filter paper and solvent evaporation. Finally, screening of analgesic activity of crude extracts on Swiss Albino mice. Place and Duration of Study: School of Science and Engineering, Department of Pharmacy, Southeast University, Bangladesh, January 2011 to August 2012. Methodology: The analgesic activity was investigated for its peripheral pharmacological actions by using acetic acid-induced writhing test in mice. Results: The Methanolic and Petroleum Ether extracts, at the dose of 200 mg/kg body weight, displayed 96.82% & 94.27% pain inhibition which was significant (p<0.001) compared to control. These results indicate that the extracts possess significant analgesic activity. Conclusion: This study suggests that the Methanolic and Petroleum Ether extract of Manilkara zapota leaves have analgesic activity in a dose dependent manner which supports it’s as an analgesic drug in folk medicine.