ABSTRACT
Objective: A prospective study aimed at isolation, biochemical identification and determination of the antibiotic resistance pattern of the bacteriologic agents causing surgical site infection in surgical wards in Sohag University hospital and confirmation of isolated MRSA by detection of mecA gene by PCR assay
Patients and Methods: This study included 100 patients suffering from nosocomial SSI and recruited from different surgical wards in Sohag University hospital in the period from October 2005 to July 2006. SSI was identified using CDC definitions. The collected samples were cultured, the isolated organisms identified, tested for their antibiotic sensitivity and mecA gene detected in the isolated MRSA strains by PCR assay
Results: Gram-negative bacteria were involved in 62 [70.5%] and Gram-positive cocci in 26 [29.5%]. Five species were isolated most frequently: Staphylococcus aureus [20], Escherichia coli [20], Pseudomonas aeruginosa [16], Proteus mirabilis [12] and Klebsiella pneumoniae [11]. Resistance to most commonly available antibiotics was moderate to very high among Gram-positive and Gram-negative isolates. Almost all Gram-negative bacteria were sensitive to imipenem and amikacin. PCR assay revealed that all the isolated Staphylococcus aureus strains [100%] were positive for mecA gene
Conclusion: It is of utmost importance to estimate the frequency of surgical site infections and identify associated risk factors in order to undertake adequate measures for their prevention and control
ABSTRACT
A statistical and comparative study was done on the prescription prescribed for asthmatic children aged from 6 months up to 12 years, either by physicians of primary health care centers [PHCCs] or physicians of the Pediatric Department in Assir Central Hospital. Data regarding the prescriptions and all medical consultants were randomly collected without the physician's knowledge. The study showed that the significant differences in the prescription writing between the physicians of the Pediatric Department of Assir Central Hospital and those working in PHCCs were both in some missing items and prescribed drugs. The missing items were significant as regards the name in the prescription of PHCCs which was 26.47%, while that in the prescription of the Pediatric Department of Assir Central Hospital was 11.61%. The difference in the missing duration of medication was 34.31% in the prescriptions of PHCCs and 17.86% in the prescription of the Pediatric Department of Assir Central Hospital. As regards the prescribed drugs, the prescription of the Pediatric Department of Assir Central Hospital contained more certain drugs than in the prescriptions of PHCCs such as antibiotics and corticosteroids. While, the prescription of PHCCs contained more drugs than that of the Assir Central Hospital such as analgesic antipyretic drugs and antihistamines