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Iranian Journal of Allergy, Asthma and Immunology. 2005; 4 (3): 125-128
in English | IMEMR | ID: emr-172883

ABSTRACT

This study was conducted to measure cell-mediated immune response in healthy Epstein Barr virus [EBV]-seropositive individuals using a tissue culture "growth inhibition" assay [regression assay] where peripheral blood lymphocytes [PBLs] were tested for their ability to inhibit the outgrowth of the autologous lymphoblastoid cell lines [LCLs]. Inhibition of the outgrowth of the autologous LCLs was seen after 4 weeks by the addition of PBLs from healthy EBV seropositive donors. The regression phenomenon was never seen when the donors of peripheral blood lymphocytes were EBV- seronegative. Regression assay showed that EBV- specific memory T cells were stable in healthy EBV seropositive over many years, which indicates the persistent nature of EBV infection

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