ABSTRACT
Twelve compounds were isolated from alcohol extracts of the rhizome of Homalomena occulta by using various chromatographic techniques including column chromatography onsilica gel and C₁₈ reverse-phase silica gel, and semi-preparative HPLC. Their structures were identified by physico-chemical properties and spectroscopic data analysis as 3α, 7α-dihydroxy-cadin-4-ene (1), 3-oxofabiaimbricatan (2), 3β, 4α-dihydroxy-7-epi-eudesm-11(13)-ene (3), integrifonol A(4), 1β, 6β-dihydroxy-7-epi-eudesm-11(13)-ene (5), 4β, 7β, 11-enantioeudesmantriol (6), epi-guaidiol (7), oplopanone(8), (-)-1β, 4β, 6α-trihydroxy-eudesmane (9),2α-hydroxyhomalomenol(10), (-)-T-muurolol (11) and hamalomenol A(12). Compounds 1-7 were obtained from the genus Homalomena for the first time and 11-12 were firstly reported from the species. Additionally, compounds 3, 5 and 8 displayed inhibitory effects against the lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse macrophage RAW264.7 cells with IC₅₀ values of 6.51, 3.25, 7.78 μmol•L⁻¹, respectively.
ABSTRACT
OBJECTIVE: To investigate the chemical constituents from the aerial parts of Artemisia lactiflora. METHODS The chemical constituents were isolated by silica gel and Sephadex LH- 20 methods, and the structures were identified by NMR and MS. RESULTS: Ten compounds were isolated and identified as follows: dehydrovomifoliol(1), 3-hydroxy-1-(4-hydroxy-3, 5-dimethoxy-phenyl)-1-propanone(2), chrysindin D(3), camelliagenin A(4), 4'-O-methylalpinumisoflavone(5), 5-hydroxy-3', 4', 6, 7, 8-penta-methoxyflavone(6), armexifolin(7), 3β-hydroxy-5α, 6α-epoxy-7-megastigmen-9-one(8), carissone(9), and(E')-3β, 4α-dihydroxyl-2-(2', 4'-hexadiynylidene)-1, 6-dioxaspiro[4, 5] decane, (10). CONCLUSION: Compounds 1-5 are obtained from genus Artemisia for the first time and 6-10 are isolated from this plant for the first time.
ABSTRACT
To study the chemical constituents of Artemisia lactiflora. The compounds were isolated by column chromatography with silica gel, C18 reverse-phase silica gel, semi-preparative HPLC, and their structures were elucidated on the basis of spectral analysis. Twelve compounds were isolated from alcohol extracts of A. lactiflora and identified as 7-hydroxycoumarin (1), 7-methoxycoumarin (2), balanophonin (3), aurantiamide (4), aurantiamide acetate (5), isovitexin (6), kaempferol-3-O-beta-D-rutinoside (7), rutin (8), caffeic acid ethyl ester (9), quercetin (10), methyl 3, 5-di-O-caffeoyl quinate (11) and methyl 3, 4-di-O-caffeoyl quinate (12), respectively. Compounds 3-12 were obtained from this plant for the first time.