ABSTRACT
The purpose of this study was to establish a suitable method for extracting cerebrospinal fluid (CSF) from C57BL/6 mice. A patch clamp electrode puller was used to draw a glass micropipette, and a brain stereotaxic device was used to fix the mouse's head at an angle of 135° from the body. Under a stereoscopic microscope, the skin and muscle tissue on the back of the mouse's head were separated, and the dura mater at the cerebellomedullary cistern was exposed. The glass micropipette (with an angle of 20° to 30° from the dura mater) was used to puncture at a point 1 mm inboard of Y-shaped dorsal vertebral artery for CSF sampling. After the first extraction, the glass micropipette was connected with a 1 mL sterile syringe to form a negative pressure device for the second extraction. The results showed that the successful rate of CSF extraction was 83.33% (30/36). Average CSF extraction amount was (7.16 ± 0.43) μL per mouse. In addition, C57BL/6 mice were given intranasally ferric ammonium citrate (FAC) to establish a model of brain iron accumulation, and the CSF extraction technique established in the present study was used for sampling. The results showed that iron content in the CSF from the normal saline control group was not detected, while the iron content in the CSF from FAC-treated group was (76.24 ± 38.53) μmol/L, and the difference was significant. These results suggest that glass micropipette vacuum technique of CSF sampling established in the present study has the advantages of simplicity, high success rate, large extraction volume, and low bleeding rate, and is suitable for the research on C57BL/6 mouse neurological disease models.
Subject(s)
Mice , Animals , Vacuum , Mice, Inbred C57BL , Cisterna Magna , Brain , Cerebrospinal FluidABSTRACT
Objective To analyze the diagnostic value of apolipoprotein E and lipid levels in patients for early diagnosis of cerebral infarction.Methods A total of 80 patients with cerebral infarction were enrolled in this study as the study group from June 2015 to October 2016.Meanwhile,80 healthy subjects were selected as the control group.The serum apolipoprotein E and blood lipid were compared between the two groups.Results The difference of apolipoprotein E levels between the two groups was statistically significant (P ＜ 0.05).Apolipoprotein E combined with serum total cholesterol and low density lipoprotein cholesterol for the diagnosis of cerebral infarction had a sensitivity of 37.5 ％,specificity accuracy of 100％,and diagnostic efficiency of 60％.Conclusion Early cerebral infarction patients diagnosed with apolipoprotein E combined with lipid levels had a good clinical value.