1.
Journal of Zhejiang University. Science. B
;
(12): 1148-1152, 2005.
Article
in English
| WPRIM
| ID: wpr-263247
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.</p><p><b>METHODS</b>To measure the concentration of theophylline (n=122) and evaluate the assay.</p><p><b>RESULTS</b>The linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).</p><p><b>CONCLUSION</b>This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.</p>