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Objective:To explore the application value of soluble tumor suppressor 2 (SST2), galectin-3 combined with magnetic resonance multimodality in the diagnosis of myocardial fibrosis.Methods:The clinical data of 88 patients with cardiomyopathy from January 2017 to December 2019 in Handan Central Hospital of Hebei Province were retrospectively analyzed as the experimental group, and 100 healthy people in the same period were selected as the control group. According to the results of cardiac magnetic resonance imaging (CMRI)-late gadolinium enhanced (LGE), the patients with cardiomyopathy were divided into LGE positive and LGE negative. The arrhythmia rate was evaluated by ambulatory electrocardiogram. The New York Heart Association (NYHA) cardiac function grade was recorded. The left ventricular ejection fraction (LVEF) and left ventricular end diastolic diameter (LVEDD) were detected by echocardiography. The SST2, galectin-3 and N-terminal pro-brain natriuretic peptide (NT-proBNP) were detected by enzyme-linked immunosorbent assay (ELISA). The receiver operating characteristic (ROC) curve was drawn to analyze the efficacy of SST2 and Galectin-3 in predicting myocardial fibrosis in patients with cardiomyopathy.Results:CMRI-LGE results of 88 patients with cardiomyopathy showed that LGE was positive in 42 cases and negative in 46 cases. The arrhythmia rate, LVEDD, SST2 and galectin-3 in experimental group were significantly higher than those in control group: 67.05% (59/88) vs. 2.00% (2/100), (46.55 ± 5.99) mm vs. (27.92 ± 2.05) mm, (61.83 ± 10.57) μg/L vs. (24.99 ± 7.69) μg/L and (18.65 ± 3.39) μg/L vs. (7.12 ± 1.33) μg/L, the LVEF was significantly lower than that in control group: (55.11 ± 8.36)% vs. (68.83 ± 9.45)%, and there were statistical differences ( P<0.01). The arrhythmia rate, NYHA cardiac function grade, LVEDD, SST2 and galectin-3 in patients with LGE positive were significantly higher than those in patients with LGE negative: 88.10% (37/42) vs. 47.83% (22/46), (3.10 ± 0.53) grade vs. (2.11 ± 0.61) grade, (48.88 ± 5.95) mm vs. (44.41 ± 5.24) mm, (65.58 ± 11.73) μg/L vs. (58.40 ± 8.10) μg/L and (21.00 ± 2.72) μg/L vs. (16.51 ± 2.39) μg/L, the LVEF was significantly lower than that in patients with LGE negative: (52.15 ± 8.23)% vs. (57.82 ± 7.60)%, and there were statistical differences ( P<0.01). ROC curve analysis result showed that the optimal critical values of serum SST2 and galectin-3 for predicting myocardial fibrosis in patients with cardiomyopathy were 65.07 μg/L and 18.46 μg/L, the area under the curve was 0.714 (95% CI 0.604 to 0.825, P = 0.001) and 0.894 (95% CI 0.828 to 0.960, P = 0.001), the sensitivity was 61.9% and 85.7%, and the specificity was 80.4% and 82.6%. Conclusions:Magnetic resonance multimodality has a good ability in detecting myocardial fibrosis, and serum SST2 and galectin-3 have good predictive value for myocardial fibrosis. SST2 and galectin-3 combined with magnetic resonance multimodality has important clinical significance in the diagnosis of myocardial fibrosis.
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As an interface between external electronic devices and internal neural nuclei, microelectrodes play an important role in many fields, such as animal robots, deep brain stimulation and neural prostheses. Aiming at the problem of high price and complicated fabrication process of microelectrode, a microelectrode twisting machine based on open source electronic prototyping platform (Arduino) and three-dimensional printing technology was proposed, and its microelectrode fabrication performance and neural stimulation performance were verified. The results show that during the fabrication of microelectrodes, the number of positive twisting turns of the electrode wire should generally be set to about 1.8 times of its length, and the number of reverse twisting rings is independent of the length, generally about 5. Moreover, compared with the traditional instrument, the device is not only inexpensive and simple to manufacture, but also has good expandability. It has a positive significance for both the personalization and popularization of microelectrode fabrication and the reduction of experimental cost.
Subject(s)
Equipment Design , Microelectrodes , Printing, Three-DimensionalABSTRACT
ObjectiveTo investigate the combined effects of one to one whole process companion delivery model on emotional status and postnatal recovery of parturients.Methods350 cases adopted one to one whole process companion delivery model were set as the observation group,another 350 cases who did not received companion delivery model were set as the control group.The emotional state and postpartum recovery (the amount of vaginal bleeding after childbirth,time of the uterus return to normal size,and the incidence of urinary retention 6 h after delivery,colostrum time and fasting time) were compared between the two groups.ResultsIn the observation group,312 cases were mild anxiety(89.1％),24 cases were with moderate anxiety (6.9％),14 cases were with severe anxiety (4.0％).In the control group,32 patients with mild anxiety (9.1％),237 cases were with moderate anxiety(67.7％),81 patients were with severe anxiety(23.2％),the difference was significant.In the observation group,postpartum vaginal bleeding was(48.6 ± 9.7) ml,time length of the size of the uterus return to normal was(4.5 ± 3.1 ) weeks,the incidence of postpartum urinary retention 6 hours after delivery was 1.4％,colostrum time (16.4 ± 5.3) h,and fasting time was (12.4 ± 2.1 ) h.In the control group,postpartum vaginal bleeding was (56.5 ± 11.2) ml,time length of the size of the uterus return to normal was(7.1 ± 4.2) weeks,the incidence of postpartum urinary retention 6 hours after delivery was 6.9％,colostrum time was (23.5 ± 4.7) h,and fasting time was (15.3 ± 2.6) h,the difference was significant.Conclusions One to one whole process companion delivery model helps reduce maternal anxiety,fears,reduce the incidence of postpartum unfavorable situation effectively and promote early resumption of maternal,it is worthy of clinical application.
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<p><b>OBJECTIVE</b>To optimize the extraction conditions for semi-bionic extraction of Guizhi Fuling pill decoction.</p><p><b>METHOD</b>The best extraction condition of the semi-bionic extraction for Guizhi Fuling pill decoction was optimized by uniform design was optimized with paeoniflorin, cinnamic acid, laetrile, pachymic acid, total polysaccharose and dry extract as the indexes.</p><p><b>RESULT</b>The optimized extraction condition is that extracting for 3 times with solvent pH values of 4.98, 7.35 and 8.00, respectively, and the total extracting time was 4.99 h.</p><p><b>CONCLUSION</b>In consideration of manufacturing fact, the pH value of thrice extractions is set at 5.0, 7.5, 8.0 respectively, and the extracting time is 2.0, 1.5, 1.5 h in turn.</p>
Subject(s)
Drugs, Chinese Herbal , Chemistry , Hydrogen-Ion ConcentrationABSTRACT
Objective To investigate the anticancer mechanism of Herba Hedyotis Diffusae on PG cell by observing the action of Herba Hedyotis Diffusae in inducing apoptosis of PG cells. Method After treating PG cells with different doses of drug containing serum of Herba Hedyotis Diffusae, FCM was used to detect the apoptosis of PG cell. Result After treating PG cell with Herba Hedyotis Diffusae, typical apoptosis characteristics could be seen and apoptosis rates were increased with the relation of dosage-effect, which were 6.86%, 9.77% and 11.69%, respectively. Conclusion Inducing the apoptosis of tumor cells maybe part of the anti-tumor mechanism of Herba Hedyotis Diffusae.