ABSTRACT
ObjectiveTo analyze the fingerprint of six pungent herbs based on the molecular connectivity index（MCI）and the matching frequency total statistical moment method， and to study the division and integration of the "imprinting template" of their volatile components， so as to find the common "imprinting template" characteristics of the pungent herbs. MethodThe volatile components of six pungent herbs were extracted by steam distillation， and their fingerprints were established by gas chromatography-mass spectrometry（GC-MS） with a programmed temperature increase（80 ℃ for 5 min， 5 ℃·min-1 to 200 ℃ for 5 min， 2 ℃·min-1 to 230 ℃ for 10 min）， a splitting ratio of 20∶1， an electron bombardment ion source（EI） and the detection range of m/z 35-650， and the average MCI and total statistical moment parameters of the fingerprints were calculated. Then the matching frequency method was used to classify， integrate and confirm the chromatographic peaks of the fingerprints of six pungent herbs. ResultThe average zero order， first-order and second-order MCI values of the volatile components of Pogostemonis Herba， Artemisiae Argyi Folium， Atractylodis Rhizoma， Asari Radix et Rhizoma， Magnoliae Flos and Schizonepetae Herba were 9.02， 5.28 and 5.05， respectively. The average values of peak number， total zero-order moment， total first-order moment and total second-order moment were 60， 169×107， 22.49 min and 36.82 min2， respectively. The 20 integrated imprinting templates were obtained by the matching frequency method for the six pungent herbs， among which three were common imprinting templates with the retention times of （25.97±0.21），（26.90±0.20），（31.64±1.24） min， respectively， and the representative components were valencene，β-elemene， caryophyllin， etc. ConclusionMCI combined the matching frequency total statistical moment can divide and integrate the characteristics of imprinting templates of six pungent herbs， and find their common chromatographic imprinting characteristics， which can provide a reference for the determination of effective substances of pungent herbs.
ABSTRACT
OBJECTIVE:To explore the improvement effect of different polar parts of Sanye Chongcha on models rat with ul-cerative colitis(UC). METHODS:The total extract was obtained from Sanye Chongcha by extraction with 80% ethanol,and dis-persed in water,from which the extracts of corresponding parts were obtained after extraction successively with trichloromethane and ethyl acetate and drying. 60 rats were randomized into normal group,model group,positive group(sulfasalazine enteric-coated tablets,312.5 mg/kg),trichloromethane extraction part group(70 mg/kg),ethyl acetate extraction part group(35 mg/kg)and wa-ter extraction part group(320 mg/kg). The rats except for normal group received 2,4,6-trinitrobenzenesulfonic acid solution(TN-BS)to establish UC model. 24 h later,the rats in the drug administration groups were given corresponding drugs ig,while those in normal group and model group were given normal saline ig,once a day,for 14 consecutive days. For the rats,the pathological change of the colon tissue was observed,and the colon mucosa damage index(CMDI)was scored;the activity of superoxide dis-mutase(SOD)and the content of malondialdehyde(MDA)in serum were determined. RESULTS:Compared to normal group,the rats in model group had obvious UC-like lesion,higher CMDI,lower activity of SOD and higher content of MDA in serum(P<0.01). Compared to model group,the rats in the drug administration groups had the lesion which became less serious or improved and had lower CMDI score,among which those of the ethyl acetate extraction part group and the positive group demonstrated a sta-tistically significant difference(P<0.01);and the rats in the drug administration groups except for trichloromethane extraction part group had higher activity of SOD and lower content of MDA in serum,among which the activity of SOD of the ethyl acetate ex-traction part group and the activity of SOD and content of MDA of the positive group demonstrated a statistically significant differ-ence(P<0.05 or P<0.01). CONCLUSIONS:The ethyl acetate extraction part of Sanye Chongcha can reduce colonic lipid peroxi-dation and thus has obvious improvement effect on the rats with UC caused by TNBS.