ABSTRACT
By observing apoptosis and morphological changes in primary culture of neurons from cerebellar granular layer, the neurons were irradiated with EMP (electromagnetic pulses), the possible injury mechanism was explored. The changes in death and apoptosis of the neurons were detected by MTT and flow cytometry, respectively. The slides stained with HE and TUNNEL were examined with light and fluorescence microscopy. The results showed that not only death of the neurons occurred immediately, but also apoptosis appeared after irradiation with EMP. Our conclusion is EMP can promote necrosis and apoptosis of neurons from cerebellar granular layer at early stage, which may result from DNA injury induced by EMP.
ABSTRACT
By observing the changes in LDH、AST、CHE、K、Na+ contents in supernatant of cultured rat pituitary cells after electromagnetic pulse (EMP) irradiation the injury mechanism to pituitary cells was explored. Pituitary cells of Wistar rat was cultured in 6-hole plates. The cells in five plates were irradiated with high field strength EMP ( electromagnetic pulse) for 5 times within 2 minutes. The electromagnetic pulse simulator provided a high electric field intensity of 60 KV/m, with rise time 20-nsec and pulse wide 30/?S. Contents of LDH, AST, CHE, K+ and Na+ in the supernatant were measured using reagent boxes (Beijing Zhongsheng High-tech Bioengineering Company) at Oh, 1h, 6h, 12h and 24h following irradiation, respeetively. All data were analyzed by statistical software Spss 8. 0. The results showed that LDH and CHE contents in the supernatant increased significantly at Oh after irradiation with EMP (P 0. 05). Our conclusion is EMP could injure membranes of pituitary cells.
ABSTRACT
Eleven measurements of human up palates from two hundred invividuals were analyzed by the discriminant analysis in order to determine sex.Statistical analysis of the up palate measurements on the sexual dimorphism showed that ten measurements have sex difference(P
ABSTRACT
The pathological changes of pulmonary fat emdoiksm were studied in ratsby means of an intravenous injection with autosnbcutaneous fat.The resultsshowed that most fat ernboli appeared in pulmonary arterioles and capillariesafter 24 hours.By 4 days,large number of sudan Ⅲ-positive emboli were seenthroughout the lungs.The inflammatory cellular infiltration including the histoc-ytes,macrophages,polymorphonuclear leucocytes and occasional eosinophils,pulmonary edema and focal hemorrhage were observed.After 7 days,most fatemboli disappeared.No fat embolism in other organs was found.