ABSTRACT
Objective:To establish a method for the determination of tanshinoneⅡA and salvianolic acid B in Zhiyou capsules by HPLC. Methods:The chromatographic column was Waters Atlantis@T3(150 mm × 4. 6 mm,3 μm)using acetonitrile and aqueous ammonium acetate solution(adjusting pH to 2. 4 with formic acid)as the mobile phase with gradient elution,the flow rate was 1. 0 ml ·min-1 and the detection wavelength was 260 nm. Results:The contents of salvianolic acid B and tanshinoneⅡA showed good linear relationship within the range of 1. 20-60. 00μg·ml-1(r=0. 999 7)and 0. 40-20. 00 μg·ml-1(r=0. 999 8),respectively. The av-erage recovery was 101. 53%(RSD=1. 14%)and 95. 17%(RSD=4. 33%),respectively. Conclusion:The method is efficient,con-venient and accurate,which can be used in the quality control of Zhiyou capsules.