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Objective To study complement receptor typeⅠ(CR1)activity of erythrocytes in pa-tients with SLE.Methods Using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),CR1rosette(RBC-CR1R)and immuno-complex rosette(RBC-ICR)on red cell,the ery-throcyte complement receptor I type(ECR1)genomic density polymorphism(HH type,HL type,LL type)and erythrocyte CR1immune activity were determined in32patients with SLE and in48normal individuals.Results It was found that HH type rate of ECR1density polymorphism in patients with active SLE was significantly lower(10/16,62.5%)than that(13/16,81.3%)in patients with stable SLE.The level of CR1immune activity in HH type was significantly higher than that in HL,LL type of SLE,and significantly dif-ferent from that in48normal individuals(P
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Objective To study the relationship between innate immunity of lymphocytes and hu-moral and cell-mediated immunity in patients with psoriasis.Methods The rapid natural immune reaction on cancer cells was measured by lymphocytes isolated from fresh blood.Serum IgG,IgA,IgM were measured with rate-nephelometry in patients with psoriasis,and sandwich ELISA technique was applied to detect serum sIL-2R level in those patients.Results Rosette formation rate of lymphocytes adhering to cancer cells and serum immunoglobulin IgA level were significantly higher in patients with psoriasis than those in normal controls(P
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Objective To study CD35on erythrocytes and erythrocyte chemokine recepter(ECKR)in patients with psoriasis in order to explore red blood cell(RBC)innate immune function and its possible role in the pathogenesis of psoriasis.Methods The rapid natural immune reaction on cancer cells was measured with RBCs isolated from fresh blood.Expression of CD35on erythrocytes was detected by flow cy-tometry.The level of IL-8,which was bound to isolated erythrocytes,was measured by enzyme linked im-munosorbent assay(ELISA)in the supernatant after centrifugation,which represented ECKR binding activity.Results Rosette formation rates of RBC adhering to cancer cells and expression of CD35on RBCs were significantly higher in psoriatic patients than those in control group(P
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This paper discussed anti blood group antibody and red blood cell innate immunological function changes in gravidas with various blood groups. The 1∶64 positive rate of anti blood group antibody and innate immunological adhesive ratio of CR1 on red blood cell of gravida were assayed. Results showed that gravida with blood group AB didnot yield anti blood group antibody for antagonizing her husband blood group.However,the anti blood group antibody positive rate of gravida with blood group O was topmost(18.5%), which was also comparatable to group A (8.7%) and group B (3.0%)respectively with significant difference ( P
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Objective To determine the effect of antigen on the main immunological reaction route of red blood cells and while blood cells. Methods Cancer cells (5?10~6/ml) and/or Bacillus calmette-Guerin(BCG 0.1mg) or yeast cells(5?10~8/ml) were added into 0.2ml of whole blood cells (or 0.2ml of white blood cells) and 0.3ml of fresh plasma (or 0.3ml of NS) treated by citric acid, and incubated for 1h at 37℃. IL-8 level was measured by ELASA. The data could be divided into 4 groups. (1) 0.2ml of antigen (cancer cells or yeast cells or BCG) was added to 0.2ml of whole blood cells and 0.3m plasma. ②0.2ml of NS was added to 0.2ml of whole blood cells and 0.3ml of plasma. ③ 0.2ml of antigen was added to 0.2ml of white blood cells and 0.3ml of plasma. ④ 0.2ml of NS was added to 0.2ml of white blood cells and 0.3ml of plasma. Results Cancer cells, BCG and yeast cells could activate immunological reaction in blood, but could not activate immunological reaction of white blood cells in no plasma group with addition of antigen. The activation Index (2.124?0.860) of IL-8 in the group with addition of whole blood cells and plasma was significantly higher than that (0.390?0.08) in the group with addition of antigen, white blood cells and plasma (P