Study on the immunogenicity and the stability of the toxicity,antigenicity of Neisseria meningitidis serogroup A/C / 中华微生物学和免疫学杂志

Objective To study the stability of the toxicity,antigenicity and immunogenicity of Neisseria meningitidis serogroup A/C[CMCC(B)29201/29205],and to analyze the quality of the capsular polysaecharide extracted from Neisseria meningitidis.Methods The toxicity of the 3rd,5th,10th,15th,20th,25th and 30th passage of the Neisseria meningitidis was assayed in mice,and the antigenicity of each passage was measured by the tube agglutination test(TAT)and ELISA.The effect of individual 30 passages of Neisseria nveningitidis on the brain tissue and the immunogenicity of each passage were tested in mice,and the capsular polysaccharide was purified and analyzed.Resuits The LD50 of the strains CMCC(B)29201/29205 of each passage was low(≥109/ml),and all the 30 passages of the two strains had no effect on the brain tissue of the mice.The titer of each passage was 1∶320 in TAT and more than 1∶3752 in ELISA.After immunization with individual 30 passages of the Neisseria meningitidis the titers in serum bactericidal assay (SBA)were all more than 1∶32.The capsular polysaccharide purified from Neisseria meningitidis met the quality standard of the requirement.Conclusion The strains of Neisseria meningitidis serogroup A/C used in the manufacture of the meningococcal conjugate vaccine,are stable in the toxicity,antigenicity and immunogenicity.And the capsular polysaccharide has met the quality standard.

Characteristics and Diagnosis of Diphasic Differentiated Synovial Sarcoma / 昆明医科大学学报

This paper reports a case of synovial sarcoma situated near the left sternoclavicular joint.The histological pictures of the tumor showed that the malignant epithelioid cells were in the absolute majority as cancer.Through cell culture and heterotransplantation of the tumor in vitro and in vivo,the patient's tumor,the cultured cells and the heterotransplantation tumor were observed by light microscopy,electron microscopy and histochemical stan.The features of diphasic differentiation of synovial sarcoma were identified and expounded,and it is helpful to under- standing the features of all types of synovial sarcoma and making a correct diagnosis.