Typing of fragment length polymorphism marker with primer SNP-binding at the last second 3'end / 中国法医学杂志

Objective To successfully get the full PCR alleles of the Insertion/Deletion marker rs10644346 in which a SNP-binding exists at the 3'end region of the primer.Methods Based on the AS-PCR,a common forward primer and two reverse primers with allele-specific oligonucleotides at the last second 3'end instead of the terminus were tentatively designed for typing 150 unrelated individuals and 10 father-mother--child trios from Htnan province in South-central China.Simultaneously,9 samples were typed with all the above three primers (the two primer sets which consist of the common forward primer and one of the reverse primers).Results PCR amplicons were well detected in the 150 unrelated individuals after being typed with the three primers,and the amplified fragments of parental and filial generations among 10 father-mother-child trios conformed to Mendel's principles.Allele missing was found in the two-primer group.Conclusion The primers designed by locating the specific nucleotide at the last second 3'end rather than terminal position were demonstrated also effective in getting specific alleles if perfect mismatch and PCR conditions are guaranteed,and the design strategy can provide an optional reference to rescue markers of SNP-binding primers for forensic practice.

Analysis of D1S1656 polymorphisms in Hu'nan Chinese Han population / 中国现代医生

Objective To get the genetic information and forensic parameters for the STR locus of D1S1656 in Hu'nan Chinese Han population . Methods All 451 unrelated individuals of Hu'nan Chinese Han population were selected to analyze genetic polymorphisms by using the Powerplex 21 system kit. The deviation of allele frequencies from Hardy-weinberg equilibrium was tested and the forensic parameters were counted by software. Results The marker of D1S1656 showed no significant deviation from Hardy-weinberg expectations, and 16 alleles were found with Power of Discrimination was 0.954 and Power of Exclusion 0.667. Conclusion The STR marker of D1S1656 present rich polymorphisms in Hu'nan Chinese Han population and could provide effective application in forensic field.