ABSTRACT
Objective To provide nutritional supportive scheme for patients with radiation injury through the treatment of the one exposed to Nanjing 192Ir source accident.Methods The reasonable nutrition treatment scheme was made on the basis of dietary survey and nutritional index monitoring during clinical stages of the patient,including body weight,body mass index(BMI),biochemical indexes,electrolyte,etc.,as well as metabolic cart determination of resting energy expenditure (REE).Results Patient on admission (days 5 post-irradiated) weighing 42.5 kg,172 days after the first irradiated (the first skin grafting) fell to a minimum of 36 kg,then gradually rise,hen rose back to normal range on days 383 before discharge.Normal admission hemoglobin was 135 g/L,172 d after irradiated to a minimum of 54 g/L,normal discharge;when lymphocytes admission low as 0.5 × 109/L,58 days back to normal after exposure,172 days after irradiated down to 0.4 × 109/L.Serum albumin was normal admission 41.2 g/L,172 days after irradiated down to 25.3 g/L.The normal level of serum prealbumin was 0.22 g/L,248 days to a minimum of 0.04 g/L,the basic return to normal at discharge was 0.17 g/L.Admission normal liver function,bilirubin index slightly higher,the all in one parenteral nutrition after about 2.5 months later,bilirubin and liver function indicators were gradually increased,the adjusted treatment and nutrition liver and gallbladder and other gradually returned to normal after treatment.REE and the body weight were determined by metabolic cart on days 294,308 and 342 for the energy requirements.Conclusions For patient with radiation injury,appropriate nutrition therapy is a key method for the clinical treatment and rehabilitation,which can maintain the nutritional status of patients and improve clinical treatment.
ABSTRACT
ObjectiveTo observe the effect of Shenxiong-Huayu Capsule preconditioning on cell apoptosis and the expression of c-fos、c-jun in the hippocampal CA1 area of rats with acute cerebral ischemia reperfusion injury.Methods SD rats were divided into 3 groups by completely randomized method:sham operation group(n=6),ischemia reperfusion group (model group),and Shenxiong-Huayu Capsule preconditioning group (preconditioning group).The last two groups were divided randomly into 6 h,24 h,48 h and 72 h reperfusion subgroups (each n=6).Intragastric administration for 7 days,once a day.Middle cerebral artery occlusion and reperfushion model was made by an intraluminal filament method.Cell apoptosis was detected by TUNEL method,the expression of c-fos、c-jun was observed by immunohistochemistry.Results ① The number of apoptosic cells at 6、24、48、72hin the hippocampal CA1 area ofrats was respectively (11.17±3.71、39.83 ± 5.67、48.33± 5.32 and 22.17± 3.7 1) single/High power field in the model group,and was respectively (7.83±2.04、15.00±3.58、29.50±6.89 and 10.17±2.32) single/High power field in Shenxiong-Huayu Capsule preconditioning group.Compared with the model group,the number of apoptosic cells at each time point in the hippocampal CA1 area of rats was decreased in Shenxiong-Huayu Capsule preconditioning group(P＜0.05 or P＜0.01 ).② The number ofc-fos at 6、24、48、72 h in the hippocampal CA1 area of rats was respectively (14.50± 3.45、33.67± 1.63、42.33±3.32 and 32.00 ±2.90) single/High power field in the model group,and was respectively (10.17 ± 2.93、21.50 ± 2.43、30.83 ± 3.76 and 25.17 ± 5.27) single/High power field in Shenxiong-Huayu Capsule preconditioning group.The number of c-jun at 6、24、48、72 h in the hippocampal CA1 area of rats was respectively (15.50±4.19、22.83±5.64、33.10±4.19 and 14.67±3.08) single/High power field in the model group,and was respectively (9.67± 3.63、15.67±2.73、21.26±3.63 and 9.33 ±3.61)single/High power field in Shenxiong-Huayu Capsule preconditioning group.Compared with the model group,the expression of c-fos、c-jun at each time point in the hippocampal CA1 area of rats was decreased in Shenxiong-Huayu Capsule preconditioning group (P＜0.05 or P＜0.01).ConclusionShenxiong-Huayu capsule can inhibit apoptosis by restraining the expression of c-fos、c-jun and relive cerebral ischemia reperfusion injury in rats.