ABSTRACT
Objective To investigate the expression of serotonin transporter (5-HTT) and serotonin 1A treceptor (5-HT1 A R) located in the chronic unpredictable stress (CUS)-relative brain areas (mPFC,VTA,NAc) in high and low CUS susceptibility rats,thus to unveil the possible mechanism lead to the different CUS susceptibility.Methods One hundred and fifty male Sprague-Dawley rats were randomly assigned into experiment group (n =120) and control group (n =30).Rats in experiment group were trained according to established CUS procedure.OFT and FST were used to assess the different susceptibility to CUS:high susceptibility group (H group)and low susceptibility group (L group).After the model was established,rats were scarified and cardio-perfused,and the brains were removed and sliced up coronarily.The sections including ventral tegmental area (VTA),nucleus accumben (NAc),medial prefrontal cortex (mPFC) were selected.The mRNA levels of 5-HTT and 5-HT1AR in the regions were estimated with in situ hybridization.Results The expression of 5-HTT in H group were significantly lower than that of in the control and L group in all regions (mPFC:169.20 ± 8.23 vs 143.53 ±5.31 ; Nac:177.41 ± 5.68 vs 158.65 ± 5.24 ; VTA:174.16 ± 5.61 vs 158.65 ± 4.85),and the difference between the H and L group was significant(P＜0.01) ;however,the expression of 5-HT1AR in H group were significantly higher than that of in the control and L group in all regions (mPFC:113.98 ± 7.46 vs 125.90 ± 3.30 ; Nac:112.11± 5.50 vs 125.06 ± 3.97 ;VTA:103.11 ± 6.05 vs 115.57 ± 3.19),and the difference between the H and L group was significant (P＜ 0.01).Conclusion The overexpression of 5-HT1AR and down regulation of 5-HTT in the circuit of VTA-NAc-mPFC may be the basis of the high susceptibility to CUS.
ABSTRACT
BACKGROUND:Delayed therapy widely occurs in patients with dementia praecox;therefore,it brings a series of difficulties for clinical treatment and rehabilitation.OBJECTIVE:To analyze the influential factors of delayed therapy in patients with dementia praecox.DESIGN:Cross-sectional study based on patients with dementia praecox.SETTING:The Second Affiliated Hospital of Xinxiang Medical College.PARTICIPANTS:A total of 96 patients with dementia praecox,including 52 males and 44 females,were selected from the Second Affiliated Hospital of Xinxiang Medical College from January to June 2005.Their ages ranged from 16 to 55 years and the mean age was (32.7±12.3) years.Among them,54 patients had middle-school education and 42 patients had high-school education.METHODS:A domestic inventory was used to carefully record basically clinical data of each patient.and the inventory included sex,marriage status,educational level,attack styles,home address,home environment,economic status,family history,etc.All data were classified and analyzed in details.In addition,condition of delayed therapy,which determined as the duration over 1 year from onset of psychiatric symptoms to accepted treatment,in each classification was surveyed gradually.MAIN OUTCOME MEASURES:Delayed therapeutic rate and occurent rate of related factors.RESULTS:Among 96 patients,60 patients had delayed therapy,and the delayed therapeutic rate was 62.5%.Influential factors of delayed therapy:Logistic regression analysis demonstrated that regression equation was involved in educational level,family history,attack style and economic status.Meanwhile,the standard regression coefficient was 0.332 1,0.210 1,0.190 3 and 0.101 2. CONCLUSION:Educationallevel,family history,attack style and economic status of patients with dementia praecox are risk factors of delayed therapy. It is of importance for strengthening these factors to interfere and reduce delayed therapeutic rate at an early phase.