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Aim To establish gefitinib resistant epidermal growth factor receptor(EGFR) mutant lung cancer cell lines and to explore the changes of downstream signaling pathway of EGFR. Methods Gefitinib concentration gradient method was used to induce the establishment of H3255 and HCC827 resistant cell lines, and CCK8 assay was used to detect the proliferation of gefitinib resistant cell lines H3255/GR and HCC827/ GR. Western blot was used to detect the changes of EGFR downstream signals in H3255, HCC827, H3255/GR and HCC827/GR cells. Results The proliferation of H3255/GR and HCC827/GR cells was significantly lower than that of non-drug resistant cells. The phosphorylation signal molecules p-AKT, p-ERK1/2 and p-STAT3 of H3255/GR and HCC827/ GR drug-resistant cell lines were no significantly changed compared with their non-drug-resistant cell lines. There was no significant change in the expression of p-STAT3 and p-ERKl/2 in H3255/GR cells treated with gefitinib, but the expression of p-AKT was significantly down-regulated, while gefitinib only slightly inhibited the expression of p-ERKl/2 in drugresistant H3255/GR cells. In HCC827/GR cells, p-AKT and p-STAT3 were not inhibited by gefitinib, while p-ERKl/2 was moderately inhibited. Conclusion There are significant differences in the signal mechanism of drug resistance between H3255/GR and HCC827/GR cell lines.
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The early diagnosis of children with autism spectrum disorders (ASD) is essential. Electroencephalography (EEG) is one of most commonly used neuroimaging techniques as the most accessible and informative method. In this study, approximate entropy (ApEn), sample entropy (SaEn), permutation entropy (PeEn) and wavelet entropy (WaEn) were extracted from EEGs of ASD child and a control group, and Student's -test was used to analyze between-group differences. Support vector machine (SVM) algorithm was utilized to build classification models for each entropy measure derived from different regions. Permutation test was applied in search for optimize subset of features, with which the SVM model achieved best performance. The results showed that the complexity of EEGs in children with autism was lower than that of the normal control group. Among all four entropies, WaEn got a better classification performance than others. Classification results vary in different regions, and the frontal lobe showed the best performance. After feature selection, six features were filtered out and the accuracy rate was increased to 84.55%, which can be convincing for assisting early diagnosis of autism.
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Child , Humans , Algorithms , Autism Spectrum Disorder , Classification , Diagnosis , Electroencephalography , Entropy , Support Vector MachineABSTRACT
Autism spectrum disorders (ASD) is a complex developmental disorder characterized by impairments in social communication and stereotyped behaviors. Electroencephalograph (EEG), which can measure neurological changes associated with cortical synaptic activity, has been proven to be a powerful tool for detecting neurological disorders. The main goal of this study is to explore the effects of repetitive transcranial magnetic stimulation (rTMS) on behavioral response and EEG. We enrolled 32 autistic children, rTMS group ( = 16) and control group ( = 16) and calculated the relative power of the δ, θ, α, β rhythms in each brain area by fast Fourier transform and Welch's method. We also compared Autism Behavior Checklist (ABC) scores of the patients before and after rTMS. The results showed a significant decrease in the relative power of the δ band on right temporal region and parietal region and also a decreased coherence on frontal region after rTMS intervention. The study proves that rTMS could have positive effects on behavior of attention, execution ability, and language ability of children and could reduce their stereotyped behavior and radical behavior.
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Objective To determine the expression levels of serum miR-25 in the patients with non-small cell lung cancer (NSCLC),and evaluate its clinical significance in the screening of NSCLC.Methods Serum samples from 82 untreated NSCLC patients,including 4 with TNM satge Ⅰ,10 with TNM stage Ⅱ,11 with TNM stage Ⅲ,53 with TNM stage Ⅳ and 4 with unknown stage,and 82 healthy controls with matched age and gender were collected,and the levels of miR-25 in these samples were determined by quantitative reverse transcription PCR (qRT-PCR).The diagnostic value of miR-25 in NSCLC was evaluated by the receiver operating characteristic (ROC) curve.Results Serum miR-25 levels in NSCLC patients were significantly higher than that in healthy controls (0.017 ± 0.028 vs 0.004 ±0.004,t =4.098,P ＜0.01).The area under the ROC curve (AUGROC),sensitivity and specificity of miR-25 for the diagnosis of NSCLC were 0.818 (95％ CI:0.753-0.882),70.7％ and 80.7％,respectively.In addition,the AUCROC,sensitivity and specificity of serum miR-25 for the screening of stage Ⅰ/Ⅱ NSCLC were 0.852 (95％ CI:0.728-0.976),78.6％ and 87.8％,respectively.Logistic regression analysis showed that miR-25 was an independent risk factor of NSCLC (OR =10.84,95％ CI:5.07-23.19,P ＜ 0.01).Conclusion Serum miR-25 levels in NSCLC patients increase significantly,indicating that it may be a novel molecular biomarker for the screening of NSCLC.
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Objective To analyze the significance of levels and combined detection of plasma homocysteine(Hcy),high-sensitivity C reactive protein(hs-CRP) and lipoprotein-associated phospholipase A2(Lp-PLA2) in patients with ischemic cerebral stroke(ICS).Methods A total of 140 patients with ICS were selected into ICS group,and 100 healthy individuals were recruited in the control group.The plasma levels of Hcy,hs-CRP,Lp-PLA2 were examined,then the positive rates of them were statistically analyzed.Analyzed the value of the levels and joint detection of Hcy,hs-CRP and Lp-PLA2.Results The plasma levels and the positive rates of Hcy,hs-CRP and Lp-PLA2 in ICS group were statistically higher than those in the control group(P<0.05).The area under the receiver-operating characteristic curve of combined detection of Hcy,hs-CRP and Lp-PLA2 in diagnosis of ICS was 0.949,the sensitivity was 0.850,and the specificity was 0.807,which were higher than Hcy,hs-CRP and Lp-PLA2 alone.Conclusion The plasma levels of Hcy,hs-CRP and Lp-PLA2 increase in ICS patients,and the combined detection of the three indicators might improve the early diagnostic value of ICS.
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Objective To investigate the association of Tim-1 protein expression and its gene polymorphism with nutritional parameters in patients with systemic lupus erythematosus (SLE).Methods Peripheral blood samples were collected from 126 patients with SLE.Serum Tim-1 protein levels were detected by ELISA,and the Tim-1 gene-416G＞C,-1454G＞A polymorphism was detected by PCR-RFLP.Prealbumin,ceruloplasmin,and retinol conjugated protein levels were determined by immunoturbidimetry.Ferritin and 1,25-dihydroxy vitamin D3 levels were detected by electrochemiluminescence.Results Concentrations of serum Tim-1 protein,prealbumin,ceruloplasmin,retinol binding protein,ferritin,and 1,25-dihydroxy vitamin D3 were 249.7±30.2 pg/mL,226±42 μg/mL,363±95 μg/mL,29.4± 13.2 μg/mL,355± 164 ng/mL,and 26.4-± 11.5 ng/mL,respectively.In the-416G＞C site,GG,GC,and CC genotypes accounted for 11.9％,57.1％,and 31.0％,respectively.In the-1454G＞A site,GG,GA,and AA genotypes accounted for 67.5％,26.2％,and 6.3％,respectively.The Tim-1 protein concentration did not differ significantly between the different genotypes of the-416G＞C site (F=0.575,P=0.564) or-1454G＞A site (F=1.255,P=0.289).Tim-1 level was significandy negatively correlated with prealbumin (r =-0.176,P =0.033),and positively correlated with ceruloplasmin (r =0.205,P =0.014) and 1,25-dihydroxy vitamin D3 (r=0.166,P=0.042).The serum prealbumin level decreased significantly (P=0.027) in patients harboring the GG genotype in the-1454G＞A site,whereas the serum 1,25-dihydroxy vitamin D3 level decreased significandy (P =0.024) in patients with the AA genotype in the-1454G＞A site.Conclusion Serum Tim-1 protein level and the-1454G＞A polymorphism of Tim-1 gene are associated with the nutritional function of patients with SLE.
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PURPOSE: This study was aimed to evaluate effect of the desensitizing pretreatments on the micro-tensile bond strengths (microTBS) to eroded dentin and sound dentin. MATERIALS AND METHODS: Forty-two extracted molars were prepared to form a flat dentin surface, and then they were divided into two groups. Group I was stored in distilled water while group II was subjected to a pH cycling. Each group was then subdivided into three subgroups according to desensitizing pretreatment used: a) pretreatment with desensitizer (Gluma); b) pretreatment with CO2 Laser (Ultra Dream Pluse); c) without any pretreatment. All prepared surfaces were bonded with Single Bond 2 and built up with resin composite (Filtek Z250). The micro-tensile bond test was performed. Fracture modes were evaluated by stereomicroscopy. Pretreated surfaces and bonded interfaces were characterized by scanning electron microscope (SEM). The data obtained was analyzed by two-way ANOVA (alpha=0.05). RESULTS: For both sound and eroded dentin, samples treated with desensitizer showed the greatest microTBS, followed by samples without any treatment. And samples treated with CO2 laser showed the lowest microTBS. SEM study indicated that teeth with eroded dentin appeared prone to debonding, as demonstrated by existence of large gaps between adhesive layers and dentin. CONCLUSION: Pretreatment with Gluma increased the microTBS of Single Bond 2 for eroded and sound teeth. CO2 laser irradiation weakened bond performance for sound teeth but had no effect on eroded teeth.
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Adhesives , Cytochrome P-450 CYP1A1 , Dentin , Hydrogen-Ion Concentration , Lasers, Gas , Molar , Tensile Strength , Tooth , Tooth Erosion , WaterABSTRACT
Objective: To explore how to set up the service management center of ventilators depending on department of respiratory medicine , how to perfect daily management of ventilators, how to rationally and timely allocate ventilators ,how to reduce the fault of ventilators and ensure the safe use of ventilators, and how to cost savings of purchasing ventilators. Methods: To figure out and compare data of before and after the establishment of the service management center of ventilators in 2010 and in 2012. Results: Non machine failure rate was 42.86% in 2012 and 69.35% in 2010, had remarkably decreased. At the same time, the total failures have decreased significantly. Conclusion: Setting up the service management center of ventilators depending on department of respiratory medicine have achieved timely allocation ventilators, have improved the level of medical personnel of using ventilators, have ensured the safe use of ventilators, have cost savings of purchase ventilators, have reduced the fault of ventilators.
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Objective To explore the association between genetic variants in intron 4 of the surfactant protein B(SP-B) gene and neonatal respiratory distress syndrome(NRDS) in preterm infants.Methods Using 1 ∶ 1 casc-control study method,a cohort of 52 preterm infants with NRDS and 52 preterm infants without NRDS(non-NRDS) were recruited.Within 1 week after the patients hospitalized,2 mL venous blood was collected.Genomic DNA was extracted and polymerase chain reaction(PCR) was performed to amplify the special region with conserved sequence motifs and intermotif dinucleotide repeats(CA) n in intron 4 of SP-B gene.PCR products was demonstrated by agarose gel electrophoresis and variants were identified by product size.Results A 550 bp target band could be seen in all PCR products (including NRDS group and non-NRDS group).Besides,an additional band(about 650 bp) could be seen in 3 cases of NRDS and 1 case of non-NRDS,which represented insertion variation.The variation incidence of intron 4 of SP-B gene in NRDS group and non-NRDS group were 5.77％ and 1.92％,respectively,there was significant differences (x2 =44.18,P ＜ 0.05).Conclusion Genetic variants in intron 4 of SP-B gene may contribute to risk of preterm infants with NRDS.