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Chinese Traditional and Herbal Drugs ; (24): 3573-3577, 2016.
Article in Chinese | WPRIM | ID: wpr-853208

ABSTRACT

Objective: To study the chemical constituents of Phyllanthus urinaria. Methods: Compounds were isolated and purified by the normal phase silica gel, Sephadex LH-20, MCI gel, RP-18, and semi-manufactured preparation HPLC method. Their structures were identified by the methods of 1H-NMR and 13C-NMR combined with physicochemical property. Results: Sixteen compounds were isolated from the petroleum ether and ethyl acetate fraction in 95% ethanol extract of P. urinaria and their structures were gallic acid (1), caffeic acid (2), ethyl gallate (3), methyl gallate (4), 4-ethoxybenzoic acid (5), diisobutyl phthalate (6), dibutyl phthalate (7), (4R,6R)-2,3-dihydromenisdaurilide (8), (4R,6S)-2,3-dihydromenisdaurilide (9), aquilegiolide (10), menisdaurilide (11), cassipourol (12), (9Z,12Z)-nonadeca-9,12-dienoic acid (13), methyl linoleate (14), stigmasterol (15), and (8R,8'S,7S)-4'-(3″- methoxyrhamnopyranosyl) oxy-8'-hydroxy-3,3',4-trimethoxy-8-hydroxymethyl-lign-7-9'-lactone (16). Conclusion: Compounds 5-7, 10, 11, 13, and 14 are isolated from this plant for the first time, and compounds 8, 9, 12, and 16 are first isolated from the plants in genus Phyllanthus L. for the first time.

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