Interleukin-1β and C-reactive protein level in plasma and gingival crevicular fluid in adolescents with diabetes mellitus / 北京大学学报(医学版)

OBJECTIVE@#Cytokines produced by various cells are strong local mediators of inflammation. Interleukin-1beta (IL-1β) and C-reactive protein (CRP) play essential roles in the development and progression of diabetes mellitus (DM). Thus periodontal diseases could be related to DM via the same mediators of inflammation. To evaluate plasma and gingival crevicular fluid (GCF) levels of IL-1β and CRP in adolescents with DM to further investigate whether DM has an impact on the levels of inflammation factors at an early stage, and to analyze the risk of developing periodontal diseases in adolescents with DM.@*METHODS@#A total of 121 adolescents aged from ten to sixteen years were enrolled, 41 adolescents diagnosed with diabetes mellitus were collected in the DM group, and 80 nondiabetic adolescents as the control group. The periodontal indices of each individual were recorded, including plaque index (PLI), modified bleeding index (mBI), probing depth (PD) and attachment loss (AL). GCF and intravenous blood samples were collected, and CRP and IL-1β levels were detected by enzyme-linked immunosorbent assay (ELISA).@*RESULTS@#(1) PLI of DM group and control group were 1.23±0.05 and 0.95±0.04 separately, with significant difference (P=0.001). DM group and control group had mBI of 0.80±0.08 and 0.51±0.06 separately, with significant difference (P=0.003). Attachment loss was found in none of the subjects. PDs of DM group and control group were (2.37±0.51) mm and (2.31±0.05) mm separately, and there was no significant difference. (2) CRP in GCF was only detectable in partial of the individuals, with a detectable rate of 22.9% (11/48) in total. The detectable rate of CRP in GCF was significantly higher in DM group (38.5%) than that in control group (4.5%, P=0.006). The plasma level of CRP in DM group [0.23 (0.15, 1.89) mg/L] was higher than that in control group [0.19 (0.12, 4.18) mg/L], but without significance (P=0.776). (3) The plasma levels of IL-1β in DM group and control group were (14.11±0.57) ng/L and (14.71±0.50) ng/L separately, but there was no significance (P=0.456). GCF levels of IL-1β in DM group and control group were (12.91±1.95) μg/L and (17.68±3.07) μg/L, without significant difference (P=0.185).@*CONCLUSION@#Periodontitis was not observed in adolescents with DM at an early stage. However, the rising levels of periodontal indices and CRP in GCF, might indicate that adolescents with DM have a higher risk of developing periodontal diseases in the future.

The salivary factors related to caries and periodontal disease in children and adolescents with diabetes mellitus / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To detect the salivary factors related to caries and periodontal disease and to analyze the risk of caries and periodontal disease in children and adolescents with diabetes mellitus.</p><p><b>METHODS</b>The study comprised 30 children with diabetic mellitus, aged 7-15 years old, and 60 healthy age-and gender-matched children. Caries and periodontal indexes were recorded and saliva related factors were analyzed.</p><p><b>RESULTS</b>Caries indexes of diabetes children [permanent teeth: decay missing filling tooth (DMFT) M (Q1,Q3) = 0(0, 4), deciduous teeth: decay missing filling tooth (dmft) M (Q1,Q3) = 0(0, 1)] were not significantly different with those of healthy children [DMFT M (Q1,Q3) = 1(0, 3), dmft M (Q1,Q3) = 0(0, 4)], but plaque index (PLI) (1.25 ± 0.33) and bleeding index (BI) (0.74 ± 0.45) of diabetes children were significantly higher than those of healthy children (PLI was 0.93 ± 0.31,BI was 0.34 ± 0.22) (P < 0.001). Salivary pH of diabetes children (7.68 ± 0.36) was significantly higher than that of healthy children (7.30 ± 0.32) (P < 0.05), and salivary acid buffering capacity had no significant difference between the two groups (P > 0.05). Salivary glucose, immunoglobulin sIgA and sIgG were not significantly different between the two groups (P > 0.05).Salivary lysozyme of diabetes children was significantly higher than that of healthy children (P < 0.05). Total protein was significantly lower in diabetes children than in healthy children (P < 0.05). Salivary lactate dehydrogenase had no significant difference between the two groups (P > 0.05).</p><p><b>CONCLUSIONS</b>Diabetes mellitus can lead to the changes of some salivary factors related to gingivitis in diabetes children. Children and adolescents with diabetes mellitus may have a higher risk of periodontal disease.</p>

Investigation of semen quality of 18-35 year old Chinese army men / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the semen quality of the Chinese army men.</p><p><b>METHODS</b>Ten-item sperm quality analyses were made by manual methods and the computer assisted sperm analysis system in 1054 young Chinese army men. The subjects were divided into 4 age groups (18-20 yrs., 21-25 yrs., 26-30 yrs and 31-35 yrs.), and the results of the analyses were compared.</p><p><b>RESULTS</b>Among the 1 054 young males investigated, the semen volume was (2.6 +/- 1.4) ml, sperm density (55.9 +/- 46.5) x 10(6)/ml, sperm grade a + b motility (47.1 +/- 19.0)%, sperm viability (70.6 +/- 22.1)%, morphologically normal sperm (84.7 +/- 10.2)%, and acrosomal integrity (86.1 +/- 7.2)%. As for the percentages of the quality indexes that met WHO standards, the sperm volume was 73.5%, liquefaction time 91.1%, pH 93.0%, grade a + b motility 45.5%, viability 86.7%, sperm density 80.4%, morphologically normal sperm 98.2%, and the sperm total number 78.0%. Those who accorded with all the WHO standards accounted for 40.2%.</p><p><b>CONCLUSION</b>The semen quality of the 18-35 year old army men was better than previously reported in the similar literature. And that of the 26-30 yrs. group was the best among all the age groups.</p>

Influence of varicocele on sperm chromatin structure and sperm motility / 中华男科学杂志

<p><b>OBJECTIVE</b>To study the influence of varicocele on sperm chromatin structure and sperm motility.</p><p><b>METHODS</b>Routine semen analysis and sperm chromatin structure assay (SCSA) were performed in a varicocele group (n=74) and a control group (n=89).</p><p><b>RESULTS</b>Sperm concentration (41.4 +/- 38.7] x 10(6)/ml) grade a+b sperm percentage ([31.7 +/- 16.9]% and sperm viability ([62.8 +/- 22.2]%) in the varicocele group were evidently lower than those ([80.9 +/- 63.1] x 10(6)/ml, [46.8 +/- 20.5]%, [77.2 +/- 17.5])% in the control group (P < 0.05) and so were VCL, VSL and VAP ([37.4 +/- 12.5 microm/s, [23.4 +/- 7.8] microm/s, [26.5 +/- 8.2] microm/s) in the varicocele group than those ([42.4 +/- 10.7] microm/s, [27.3 +/- 7.3] microm/s, [30.7 +/- 7.8] microm/s) in the control (P < 0.05). MAD was increased (P < 0.01), and the COMP alphat of SCSA (23.2 +/-16.2) was obviously higher in the former than in the latter (14.1 +/- 11.8) (P < 0.05).</p><p><b>CONCLUSION</b>Varicocele causes damage to sperm DNA and changes sperm motility, which may result in male infertility.</p>