ABSTRACT
Objective: To study the relationship between UPLC and anti-inflammatory effects of different extracts of Salvia miltiorrhiza, and to provide a basis for clarifying the material basis of anti-inflammatory effects of S. miltiorrhiza. Methods: UPLC was used to analyze the different extraction sites of S. miltiorrhiza. The anti-inflammatory effect of anti-bronchial epithelial cell inflammation model was studied by different extraction sites. The spectral relationship was established by gravitational correlation analysis (GRA). Results: 50% grams of ethanol extract (S3), 80% ethanol eluent (S4), filtered precipitate (S5), and extracted aqueous layer extract (S9) had strong anti-inflammatory ability to inflammatory cells. The 50% ethanol eluate (S3) of the ethanol extract of Danshen was the strongest. The effect of chemical composition on the anti-inflammatory efficacy of each characteristic peak was as the following order: A9>A1>A2>A6>A15>A11>A12>A7>A10>A5>A14>A3>A18>A4>A13>A16>A8, and the top four peaks with strong contribution to anti-inflammatory effects were peaks 9, 1, 2, and 6, respectively. The results were as follows: tanshinone IIA, danshensu, salvianolic acid B, and 3’-methyl salvianolic acid B. Conclusion: The anti-inflammatory efficacy of S. miltiorrhiza is the result of combination of various components. It is clear that the four components are tanshinone IIA, danshensu, salvianolic acid B, and 3’-methyl salvianolic acid B. The efficacy of the largest contribution to the future to explore the efficacy of anti-inflammatory effects of S. miltiorrhiza provides new ideas.