ABSTRACT
The present research was aimed to investigate the effects of sinusoidal electromagnetic fields (SEMFs) on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells in rats (rBMSCs) and to find out the intensity with the best therapeutic efficacy. Primary rat bone marrow mesenchymal stem cells were obtained from Wistar rats and screened by the adhesive method. The rBMSCs were exposed to sinusoidal electromagnetic fields with 50Hz frequency and intensities of 0 mT, 1.4 mT, 1.6 mT, 1.8 mT, 2.0 mT, and 2.2 mT respectively, 30 min per day. The proliferation of the rBMSCs was analyzed by MTT reduction assay. The osteogenic differentiation markers including ALP activity, calcium deposition, mineralized bone modulus and collagen I expression were compared between the rats in the exposed groups and those in the control group. The total cellular RNA was extracted after 6, 12, 24 and 48 hours, respectively. The gene expression of Osterix and IGF-1 was examined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The absorbance of exposed groups was suppressed significantly in comparison with that in the control group. The exposure to the rBMSCs with intensity of 1.8 mT strongly enhances the osteogenic differentiation of rBMSCs, indicated by remarkably improved ALP activity, calcium deposition, collagen I expression and the number of mineralized bone nodules compared to that in the control group and other groups. Osterix and IGF-1 were also significantly improved (P < 0.05). The SEMFs with frequency and 50Hz and 1.4-2.2 mT intensities enhanced the osteogenic differentiation of rBMSCs, but inhibited their proliferation in the presence of 0.1% serum culture. Among the rBMSCs used in the tests, the one with 1.8 mT had the strongest activity, indicating that it could be the optimal intensity for the clinical application.
Subject(s)
Animals , Rats , Alkaline Phosphatase , Metabolism , Bone Marrow Cells , Cell Biology , Radiation Effects , Cell Differentiation , Radiation Effects , Cell Proliferation , Radiation Effects , Cells, Cultured , Electromagnetic Fields , Mesenchymal Stem Cells , Cell Biology , Radiation Effects , Osteoblasts , Cell Biology , Radiation Effects , Rats, WistarABSTRACT
BACKGROUND:Tranditional Chinese medicine,which possesses anti-oxidation properties,can promote directional differentiation of mouse bone marrow mesenchymal stem cells(BMMSCs)into nerve cells.Salidrosides,as the effective constituent of Rhodiola,have strong anti-oxidation function.OBJECTIVE:To investigate the molecule mechanism of salidrosides induced differentiation of mouse BMMSCs into nerve cells.METHODS:When in vitro cultured BMMSCs reached 80% confluency,the cells were assigned into 3 groups.Cells in the control group were cultured by complete culture medium;those in the induction and positive control groups were cultured by complete culture medium adding 20 mg/L salidrosides or 0.1 mg/L nerve growth factors(NGF).The related gene and protein of nerve cells were detected using RT-PCR and Western blot method at 12 hours after culture.After that,the cells in the induction group were divided into 3 groups,the blocking agents EGTA(Ca~(2+) chelator),Nifedipine(L-type Ca~(2+) channel blocker)and LY294002(IP3 receptor blocking pharmacon)were applied to block the cellular Ca2* signal pathway respectively for 12 hours.RT-PCR and Western blot methods were used to study the signal transduction of the salidrosides.RESULTS AND CONCLUSION:①The expression of neuron specific enolase(NSE),B-Tubulin III,Nurri mRNA could be found ir the induction and positive control groups,instead of the control group;The expression abundance of the positive control group was smaller than that of the induction group.The expression abundance of GFAP mRNA was very low in each group,but the c-fos mRNA was expressed abundantly in the induction group.②Compared with the positive control group,the induction group could promote the NSE expression obviously,which was no expressed in the blank control group.?The expression of NSE and Nurri were conspicuously down-regulated when the Ecto Ca~(2+) and L-type Ca~(2+) channel and IP3 receptor were blocked respectively.Salidrosides can induce the differentiation BMMSCs into nerve cells. Ca~(2+) signaling and IP3 dependent Ca~(2+) signaling pathway play an important role in transduction salidrosides signal in BMMSCs differentiation.
ABSTRACT
This observation was to assess the treatment effects of free medial sural artery perforator muscle flap on foot reconstruction.Seven patients(6 men,and 1 woman;age 19 to 46 years,mean 33) with soft tissue defects on the foot underwent surgical procedures by using free medial sural artery perforator muscle flap.The coverage of the muscle flaps was performed by a meshed split-thickness skin graft.The donor site Was closed.Median follow-up Was 2.5 years(range 7 monks to 5.5 years).All the muscle flaps and skin grafts survived without major complications,and no morbidity was found at the donor sites.The muscle flaps seem to have advantage in blood supply,vascular anatomy and pedicle length,and may be helpful in the mpmr of soft tissue defects on the foot.
ABSTRACT
BACKGROUND: Chronic osteomyelitis is difficult to manage clinically, and two or more operations were commonly needed. No satisfactory method for one-stage repair has been currently available.OBJECTIVE: To examine the possibility of using fibrin glue(FG) as the common carrier for both bone morphogenetic protein(BMP) and gentamicin for one-stage repair of infected bone defects.DESIGN: A completely randomized controlled experiment.SETTING: Center of Orthopaedic Surgery, Lanzhou General Hospital of Lanzhou Area Command of of Chinese PLA.MATERIALS: The experiment was conducted using 48 healthy adult Chinchilla rabbits of either sex on normal diet with body mass of 1.9 to 2.4 kg,provided by the Institute of Biological Products, Lanzhou, Gansu Province.INTERVENTIONS: Animal models of chronic osteomyelitis were established in 48 Chinchilla rabbits, and after thorough debriding of the wounds,an elongated semicircular bone defect was induced 1.5 cm to the medial proximal metaphysis in the tibias. Three treatments were subsequently given with FG, MP and gentamicin complex(Group A), FG/BMP complex(Group B), and nothing(control group, Group C), respectively.MAIN OUTCOME MEASURES: The general conditions of the rabbits were observed postoperatively. Bacterial culture of the bone specimen, bacterial counting, X-ray and histological examination were performed.RESULTS: Rabbits in Group A exhibited obviously better infection control and greater regenerated bone mass than those in Group B, and no significant difference was noted in infection control between Groups B and C, the latter group showing poor bone defect repair.CONCLUSIONS: FG, BMP and gentamicin complex helps to control infection and promotes bone formation, and can be used to repair bone defects caused by infection and open injury with severe contamination.
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The single nucleotide polymorphisms (SNP) of vitamin D receptor (VDR) gene were determined by BsmI restriction endonuclease analysis and quantitative ultrasonography indexes (QUI) of calcaneum were measured in Tibetan women. The results showed that there were no statistical differences between QUI of different genetypes and between distribution frequencies of gene types in young women and menopausal women. And it was found that the SNP of VDR by BsmI restriction endonuclease analysis was not relevant to bone mass acquisition and rate of bone mass loss.
ABSTRACT
AIM To investigate the changes of biochemical index of osteoporosis in rats induced by given total flavonoids of herba epimedii (HEF) . METHODS The rat osteoporosis was induced by given retinoic acid intragastrically, the biochemical index in serum (T, E 2, ALP, PTH, BGP, Osteocalcin ) and in urine (Ca, P, DPD and Cr) were determined after the rats were given HEF by low, middle and high dosages respectively, and were compared with the model group. The normal control and the positive control. RESULTS The E 2, T and BGP level in all three dosage groups were obviously higher than that of the model group. The Ca/Cr, DPD and PTH level were obviously lower than the model group. The content of Ca and P and the bone density in femur were similar with the normal control, and there was significant difference compared with the model group. CONCLUSION HEF prevents the rats treated by retinoic acid from becoming osteoporosis. The changes of biochemical index were consistent with the variation of bone density, indicating the great significance of biochemical index in diagnosis and treatment of osteoporosis.