ABSTRACT
Objective: This study aims to evaluate the fractions from whole extracts of roots and stem barks of Newbouldia laevis and their effect on sickling. Methods: Hydroethanolic extracts of stem barks and roots of Newbouldia laevis were fractionated by the technics of cold precipitation in ethanol. The fractions obtained after phytochemical screening were subjected to Emmel test to evaluate their anti-sickling activity. Active fractions were tested for DPPH and AAPH assay (AAPH induced membrane lipoperoxidation and evaluation of reduction of hemolysis). Results: Two fractions were obtained from each whole extract: supernatant and pellet fractions. Supernatants fractions obtained from whole roots barks extract and stem barks extract at a concentration of 30 mg/ml reduced sickling up respectively to 7% and 10% against 86% for the control. Pellets fractions obtained from the both extracts induced coagulation of SS blood at 30 mg/ml against 86% for the control. Conclusion: Supernatants fractions of hydroethanolic whole extract of roots and stem backs of Newbouldia laevis promise as the potential source of active molecules against sickle cell disease.
ABSTRACT
Parkia biglobosa is one of the main medicinal plants used in traditional medicine to treat cardiovascular diseases in Africa. We investigated cardioprotective effects of stem bark hydro-alcoholic extract (HAE) of P. biglobosa in isoproterenol (ISO) induced myocardial infarction (MI). Four groups of five Wistar rats of each were used. Group I, control group, received per os (p.o) saline solution (5ml kg-1 b.w.) as vehicle daily for 15 days and was injected subcutaneously with the vehicle (5ml kg-1 b.w.) at an interval of 24 hours, on day 14 and 15. Group II referred to as infarcted rats, received vehicle p.o and was injected subcutaneously with isoproterenol (125 mg kg-1 b.w.) as in group I. Groups III and IV were pre-treated p.o with HAE (90 and 60 mg kg-1 b.w. respectively) daily for 15 days and was injected subcutaneously Isoproterenol (125 mg kg-1 b.w.). ISO has induced MI, characterized by significant increased (p <0.001) of MDA, biochemical alterations, heart hypertrophy and histological changes in infarcted rats. HAE, prevented significantly (p <0.001) induction of all these anomalies in pre-treated rats. These results suggested cardioprotective effects of P.biglobosa against ISO induced MI.
ABSTRACT
Purpose: Aloe buettneri A. Berger is commonly used in Togolese folk medicine to treat inflammation and gastric ulcer. In this study we investigated the anti-oedema; analgesic; antipyretic and ulcer healing properties of the hydro-alcohol extract of their leaves. Methods: Rat oedema paw were induced by the injection of 0.1 ml of formaldehyde 1; tail flick method is used to study analgesic property; hyperthermia was induced by subcutaneous injection of 15of a brewers' yeast suspension at dose of 10 ml/kg and ulcers were induced by ethanol or HCl/ethanol mixture. Results: The extract showed anti-inflammatory properties at doses between 250-500 mg/kg. It inhibited; in a dose- dependent manner; the oedema induced by 0.1 ml of formaldehyde 1. Scores of 73.70and 83.63were obtned when the doses of extract administered were 100 and 500 mg/kg; respectively. The tail flick analgesic index showed an increase of 36.56when the dose was 500 mg/kg. The extract decreased significantly the hyperthermia induced by the injection of yeast. 1000 mg/kg of the extract inhibited 63.77of the gastric lesion induced by acid-water-ethanol mixture while daily administration of the same dose accele- rated the cicatrisation of gastric ulcer induced by 95ethanol. Conclusion: The results obtained show that the hydro-alcohol extract of Aloe buettneri A. Berger (Lillia- ceae) has anti-inflammatory; anti-ulcer and wound healing properties