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OBJECTIVE@#To analyze the proliferation potential of bone marrow-derived mesenchymal stem cells (MSC) in patients with myelodysplastic syndrome (MDS).@*METHODS@#The MSC derived from the 24 patients with newly diagnosed MDS (MDS-MSC group) and MSC derived from 15 patients with nutritional anemia (control group) in the Affiliated Hospital of Hebei University were used as the research objects. The proliferation potential of MSC was analyzed by colony-forming unit assay, doubling time, cumulative passaging, cell number after 10 days of culture with equal amount of MSC and MTT experiment. The mechanism of abnormal proliferation was analyzed by cell cycle experiment, apoptosis experiment and p21 gene expression assay.@*RESULTS@#In the colony forming unit assay, the number of MDS-MSC colonies was 4.44±2.51, which was significantly lower than that of the control group (12.44±2.55)(P<0.01); the doubling time of MDS-MSC group was significantly longer than that of the control group (7.80±3.26 vs 3.63±0.85) (P<0.01); the number of MDS-MSC in 5×10@*CONCLUSION@#The proliferative capability of MDS-MSC is significantly reduced, which relates with the arrest of cell cycle in G
Subject(s)
Humans , Apoptosis , Bone Marrow Cells , Cell Proliferation , Mesenchymal Stem Cells , Myelodysplastic SyndromesABSTRACT
Two new triterpenoid saponins, ardisicrenoside R and S (1 and 2), and one new phenylpropanoid glycoside, ardicrephenin (3), along with five known compounds (4-8), were isolated from roots of Ardisia crenata. Their structures were elucidated on the basis of NMR spectroscopic data and chemical methods. Compounds 2-7 were evaluated for their cytotoxic activities against A549, MCF-7, HepG2 and MDA-MB-231 cell lines by MTT assay. Ardicrenin (6) showed significant cytotoxicity, with IC
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OBJECTIVE@#To explore the effect of dynamic scalp acupuncture combined with task-oriented mirror therapy (TOMT) for upper limb motor function and activity ability of daily living in patients with hemiplegia after ischemic stroke.@*METHODS@#Seventy-eight patients with hemiplegia after ischemic stroke were randomly divided into a dynamic scalp acupuncture group, a TOMT group, and a scalp acupuncture group, 26 cases in each group (1 case dropped off in the TOMT group and the scalp acupuncture group respectively). All three groups received routine rehabilitation training and medication treatment. The TOMT group was treated with TOMT for 40 min a day, 5 days a week for 8 weeks. The scalp acupuncture group was treated with TOMT after the scalp acupuncture, and the dynamic scalp acupuncture group was treated with TOMT during the scalp acupuncture treatment. The scalp acupuncture treatment was applied at ipsilateral up 1/5 and mid 2/5 of and , and the needles were retained for 40 min. The scalp acupuncture treatment was given once a day, 5 days a week for 8 weeks. Before treatment and after 4 weeks and 8 weeks of treatment, the functions of upper limb and hand were evaluated by simplified Fugl-Meyer assessment (FMA) scale upper limb part and functional test for the hemiplegic upper extremity-Hong Kong version (FTHUE-HK) grade, the muscle tension of the upper extremity flexor elbow muscle group was assessed by modified Ashworth scale (MAS) and the activity ability of daily living was assessed by modified Barthel index (MBI).@*RESULTS@#After 4 weeks and 8 weeks of treatment, the FMA scores, FTHUE-HK grades and MBI scores in the three groups were better than those before treatment (<0.01, <0.05), and MAS scores after 4 weeks of treatment in the three groups were better than those before treatment (<0.05). After 4 weeks and 8 weeks of treatment, the FMA scores, FTHUE-HK grades and MBI scores in the dynamic scalp acupuncture group were better than those in the TOMT group and the scalp acupuncture group (<0.05), and these items in the scalp acupuncture group were better than those in the TOMT group (<0.05).@*CONCLUSION@#The dynamic scalp acupuncture combined with TOMT treatment can effectively improve the upper limb function and the activities ability of daily living in patients with hemiplegia after ischemic stroke, which is better than TOMT after scalp acupuncture and simple TOMT treatment.
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Objective To investigate the correlation between Epstein-Barr virus (EBV) infection and systemic lupus erythematosus (SLE) in Chinese population by meta-analysis.Methods The following databases such as China National Knowledge Infrastructure (CNKI),Chinese Weipu Database (VIP),PubMed,EMbase,Chinese Biological Medicine Database (CBM),WANFANG Database were searched from inception to July 2017.For a study it had to be a case-control with SLE and controls in Chinese population.The study had to assay serum anti-EBV antibody in each group.Quality assessments were performed using a modified version of the Newcastle-Ottawa scale (NOS).Then meta-analysis was performed using Rev Man 5.3 software and Stata 12.0 software.Mantel-Haenszel odds ratios (OR) for the detection of anti-EBV antibodies were calculated as well as stability evaluated by sensitivity analysis.Egger test was used to evaluate the publication bias.Resuits Seventeen case-control studies were included.These studies included a total of 1 392 SLE cases and 1 647 controls.Twelve studies measured anti EBV viral capsid antigen (EBV VCA) IgG,there was a statistically significant higher seroprevalence of anti-EBV-VCA IgG (P=0.003) in SLE cases compared to controls.Meta-analysis revealed an overall OR of 2.97 (95％ CI=1.43～6.15).Our subgroup analyses failed to demonstrate a significant difference between any of the subgroups considered.The Egger test showed that there was no significant publication bias.Seven studies measured anti-EBV VCA antibodies of the IgA and six studies assayed anti EBV-VCA antibodies of the IgM subclass,Meta-analysis showed a significant association between anti-EBV-VCA IgA,anti-EBV VCA IgM and SLE,with OR =7.01 (95％ CI =4.61 ～10.66),9.34(95％ CI=4.29～20.34) respectively.The percentage of SLE patients positive for anti EBV-VCA IgA and anti-EBV VCA IgM (37.5 ％ and 28.5 ％,respectively) were significantly lower compared with anti-EBV-VCA IgG (90.3 ％).Meta-analysis showed overall OR=1.01 (95％ CI=0.61 ～ 1.67) indicating that the difference in seropositivity rates between SLE and control groups for anti EBV-nuclear antigen1 (EBNA1) IgG was not significant (P =0.97).Conclusion The findings support the hypothesis that infection with EBV predisposes to the development of SLE.