ABSTRACT
The aim of the present study was to investigate the role of ferroptosis in acute lung injury (ALI) mouse model induced by oleic acid (OA). ALI was induced in the mice via the lateral tail vein injection of pure OA. The histopathological score of lung, lung wet-dry weight ratio and the protein content of bronchoalveolar lavage fluid (BALF) were used as the evaluation indexes of ALI. Iron concentration, glutathione (GSH) and malondialdehyde (MDA) contents in the lung tissues were measured using corresponding assay kits. The ultrastructure of pulmonary cells was observed by transmission electron microscope (TEM), and the expression level of prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA was detected by quantitative polymerase chain reaction (q-PCR). Protein expression levels of glutathione peroxidase 4 (GPX4), ferritin and transferrin receptor 1 (TfR1) in lung tissues were determined by Western blot. The results showed that histopathological scores of lung tissues, lung wet-dry weight ratio and protein in BALF in the OA group were higher than those of the control group. In the OA group, the mitochondria of pulmonary cells were shrunken, and the mitochondrial membrane was ruptured. The expression level of PTGS2 mRNA in the OA group was seven folds over that in the control group. Iron overload, GSH depletion and accumulation of MDA were observed in the OA group. Compared with the control group, the protein expression levels of GPX4 and ferritin in lung tissue were down-regulated in the OA group. These results suggest that ferroptosis plays a potential role in the pathogenesis of ALI in our mouse model, which may provide new insights for development of new drugs for ALI.
Subject(s)
Animals , Mice , Acute Lung Injury , Pathology , Apoptosis , Bronchoalveolar Lavage Fluid , Chemistry , Cyclooxygenase 2 , Metabolism , Ferritins , Metabolism , Glutathione , Glutathione Peroxidase , Metabolism , Iron , Iron Overload , Lung , Cell Biology , Pathology , Malondialdehyde , Microscopy, Electron, Transmission , Mitochondrial Membranes , Oleic AcidABSTRACT
Objective:To develop a catheter of movable tracheal cannula for single lung ventilation so as to compensate the existed disadvantages of using double-lumen bronchial catheter and bronchial occlusive device in single lung ventilation in present.Methods: A long tracheal catheter was installed into a short endotracheal catheter so as to form a complete catheter of tracheal cannula. The internally installed catheter could be rotated and moved in the cannula. After then, the front-end direction of internally installed catheter was chose through rotated the catheter according to the requirement of operation, and the single lung ventilation could be achieved when the internally installed catheter was pushed forward and was inserted in left or right bronchus. After the operation, the internally installed catheter exited out from bronchus to trachea and was used to implement double lung ventilation, or it was pulled out from outer cannula and the double lung ventilation was implemented by using outer cannual.Results: The ventilation effect of single-lung was doubtless when the catheter of movable tracheal cannula was inserted in left or right bronchus by using internally installed catheter. The technique of intubation tube was simpler than that of double-lumen bronchial catheter and bronchial occlusive device. The internally installed catheter could rotate in the outer cannula so as to protect tracheal wall and avoid oxygen deficit of patient during the processes of replacing tracheal catheter and the difficulty of replacing tracheal catheter post-operation of double-lumen bronchial catheter. Besides, it also could avoid the injury risk of anastomotic stoma of esophageal surgery caused by mis-insertion of tracheal catheter in esophagus.Conclusion: This kind of removable tracheal cannula has reasonable design, simple operation of intubation and better clinical practicability, and its feasibility is better that the product is transformed.
ABSTRACT
Objective:To develop a catheter of movable tracheal cannula for single lung ventilation so as to compensate the existed disadvantages of using double-lumen bronchial catheter and bronchial occlusive device in single lung ventilation in present.Methods: A long tracheal catheter was installed into a short endotracheal catheter so as to form a complete catheter of tracheal cannula. The internally installed catheter could be rotated and moved in the cannula. After then, the front-end direction of internally installed catheter was chose through rotated the catheter according to the requirement of operation, and the single lung ventilation could be achieved when the internally installed catheter was pushed forward and was inserted in left or right bronchus. After the operation, the internally installed catheter exited out from bronchus to trachea and was used to implement double lung ventilation, or it was pulled out from outer cannula and the double lung ventilation was implemented by using outer cannual.Results: The ventilation effect of single-lung was doubtless when the catheter of movable tracheal cannula was inserted in left or right bronchus by using internally installed catheter. The technique of intubation tube was simpler than that of double-lumen bronchial catheter and bronchial occlusive device. The internally installed catheter could rotate in the outer cannula so as to protect tracheal wall and avoid oxygen deficit of patient during the processes of replacing tracheal catheter and the difficulty of replacing tracheal catheter post-operation of double-lumen bronchial catheter. Besides, it also could avoid the injury risk of anastomotic stoma of esophageal surgery caused by mis-insertion of tracheal catheter in esophagus.Conclusion: This kind of removable tracheal cannula has reasonable design, simple operation of intubation and better clinical practicability, and its feasibility is better that the product is transformed.