Inhibition of growth and metastasis of human gastric cancer implanted in nude mice by the angiogenesis inhibitor endostatin / 中华外科杂志

<p><b>OBJECTIVE</b>To study the effects of angiogenesis inhibitor endostatin on the growth and metastasis of gastric cancer in vivo.</p><p><b>METHODS</b>Metastatic model simulating human gastric cancer was established by orthotopic implantation of histologically intact human tumor tissue into gastric wall of nude mice. Endostatin was administered sc at dose of 0 mg/kg, 2.5 mg/kg, 10.0 mg/kg and 20.0 mg/kg every other day for seven weeks. Eight weeks after implantation, the tumor size and inhibition rates and intratumoral microvessel density (MVD) and apoptotic index (AI) and the presence of metastasis are evaluated respectively after the mice were sacrificed.</p><p><b>RESULTS</b>Compared with the untreated controls, growth of the orthotopically implanted tumor was significantly reduced in size in mice treated with endostatin with an inhibition rate 0, 62.7%, 95.8% and 99.9% at the dosage of 0 mg/kg, 2.5 mg/kg, 10.0 mg/kg, and 20.0 mg/kg, respectively. The MVD was also decreased significantly in the treated mice [(13.7 +/- 3.90) versus (5.73 +/- 2.36), (2.17 +/- 1.28) and (0.66 +/- 0.25)]. The AI was increased significantly in the treated mice [(3.91 +/- 2.58)%, versus (6.76 +/- 5.03)%, (18.92 +/- 6.75)% and (28.57 +/- 10.34)%]. The incidences of peritoneal metastases was also significantly inhibited in the treated mice (87.1% versus 54.5%, 16.7% and 0). The incidences of liver metastases was also significantly inhibited in the treated mice (83.9% versus 27.3%, 8.3% and 0). The growth and metastasis of human gastric cancer implanted in nude mice were significantly inhibited in a dose-dependent manner (P < 0.05).</p><p><b>CONCLUSIONS</b>Angiogenesis inhibitor endostatin can induce apoptosis in gastric cancer by inhibiting tumor angiogenesis and has strong inhibitory effect both on tumor growth and metastasis of human gastric cancer implanted in nude mice.</p>

The diagnosis and surgical treatment of slow transit constipation of the colon / 中国普通外科杂志

Objective To evaluate the intraluminal manometry of the colon and X ray examination of colon transport in the diagnosis of slow transport constipation (STC).[WT5”HZ] Methods [WT5”BZ] 46 patients and 25 healthy controls underwent X ray examination of colon transport function, and colonic intraluminal manometry. 29 patients received colectomy including total in 15, partial in 12, and ileo sigmoidocolostomy in 2. [WT5”HZ] Results [WT5”BZ] It was found that the marker of transport function test within the colon was held for much longer time than normal controls and the colonic intraluminal pressure was much lower in patients than in controls.[WT5”HZ]Conclusion [WT5”BZ] Intraluminal manometry of the colon and X ray examination of colonic transport function test help in establishing the diagnosis of STC. Total or partial colectomy effected a cure for STC.