ABSTRACT
Angiogenesis is a crucial process in the development of inflammatory diseases, including cancer, psoriasis and rheumatoid arthritis. Recently, several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model, and the results indicated that 1-methoxycarbony-β-carboline (MCC) could effectively inhibit blood vessel formation. In this study, we further confirmed that MCC can inhibit, in a concentration-dependent manner, the viability, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro, as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo. In the zebrafish xenograft assay, MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells. The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins, including ANG, EGF, bFGF, GRO, IGF-1, PLG and MMP-1. In addition, the expression of two key membrane receptor proteins in angiogenesis, TIE-2 and uPAR, were also down-regulated after MCC treatment. Taken together, these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.
Subject(s)
Animals , Humans , Angiogenesis Inhibitors , Chemistry , Pharmacology , Carbolines , Chemistry , Pharmacology , Cell Movement , Cell Proliferation , Epidermal Growth Factor , Genetics , Metabolism , Fibroblast Growth Factors , Genetics , Metabolism , Human Umbilical Vein Endothelial Cells , Cell Biology , Metabolism , Insulin-Like Growth Factor I , Genetics , Metabolism , Neovascularization, Physiologic , Picrasma , Chemistry , Plant Extracts , Chemistry , Pharmacology , Receptor, TIE-2 , Genetics , Metabolism , Zebrafish , EmbryologyABSTRACT
Angiogenesis is a crucial process in the development of inflammatory diseases, including cancer, psoriasis and rheumatoid arthritis. Recently, several alkaloids from Picrasma quassioides had been screened for angiogenic activity in the zebrafish model, and the results indicated that 1-methoxycarbony-β-carboline (MCC) could effectively inhibit blood vessel formation. In this study, we further confirmed that MCC can inhibit, in a concentration-dependent manner, the viability, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro, as well as the regenerative vascular outgrowth of zebrafish caudal fin in vivo. In the zebrafish xenograft assay, MCC inhibited the growth of tumor masses and the metastatic transplanted DU145 tumor cells. The proteome profile array of the MCC-treated HUVECs showed that MCC could down-regulate several angiogenesis-related self-secreted proteins, including ANG, EGF, bFGF, GRO, IGF-1, PLG and MMP-1. In addition, the expression of two key membrane receptor proteins in angiogenesis, TIE-2 and uPAR, were also down-regulated after MCC treatment. Taken together, these results shed light on the potential therapeutic application of MCC as a potent natural angiogenesis inhibitor via multiple molecular targets.
Subject(s)
Animals , Humans , Angiogenesis Inhibitors , Chemistry , Pharmacology , Carbolines , Chemistry , Pharmacology , Cell Movement , Cell Proliferation , Epidermal Growth Factor , Genetics , Metabolism , Fibroblast Growth Factors , Genetics , Metabolism , Human Umbilical Vein Endothelial Cells , Cell Biology , Metabolism , Insulin-Like Growth Factor I , Genetics , Metabolism , Neovascularization, Physiologic , Picrasma , Chemistry , Plant Extracts , Chemistry , Pharmacology , Receptor, TIE-2 , Genetics , Metabolism , Zebrafish , EmbryologyABSTRACT
<p><b>OBJECTIVE</b>To develop a HPLC method for determination of triptolide in tripterygium total terpenoids tablets.</p><p><b>METHOD</b>A Lichrospher CN column was used with ethanol and water for gradient elution. The detection wavelength was set at 255 nm.</p><p><b>RESULT</b>The linear relationship of the concentrations and peak areas was good in range of 0.742-59.4 microg x mL(-1) (r = 0.9998). The average recovery was 99.2%, RSD% = 1.7%.</p><p><b>CONCLUSION</b>The method is simple, rapid and accurate and can be used for quality control of the tablets.</p>