Effect of boschniakia rossica polysaccharides on hypoxia/reoxygenation-induced cardiomyocyte damage by down-regulating miR-302a-3p expression / 中国药理学通报

Aim To explore the effect of boschniakia rossica polysaccharides ( BRPS ) on cardiomyocyte damage induced by hypoxia/reoxygenation (H/R) and its possible mechanism. Methods H/R was used to induce rat cardiomyocyte H9c2 to establish a cell inju¬ry model, and different doses of BRPS were used to treat H9c2 cells. ELISA method was used to detect the level of MDA and the activity of SOD and GSH-Px. Flow cytometry was used to detect the rate of apopto-sis. qRT-PCR was used to detect the expression of miR-302a-3p. anti-miR-NC and anti-miR-302a-3p were respectively transfected into H9c2 cells and then subjected to H/R treatment. miR-NC and miR-302a-3p mimics were respectively transfected into H9c2 cells and treated with 100 mg • L

Study of surgery combined with 125I brachytherapy for adenoid cystic carcinoma of oral and maxillofacial region / 北京大学学报(医学版)

OBJECTIVE@#To retrospectively analyze the results of treatment outcome by surgery combined with 125I brachytherapy and correlative factors of adenoid cystic carcinoma (ACC).@*METHODS@#In the study, 75 patients with primary ACC of oral and maxillofacial region were treated by surgery combined with 125I seeds brachytherapy. Radical resection or subtotal resection was applied for the tumor. The brachytherapy treatment planning system was used to create implant plans with the prescribed dose of 60 Gy to 120 Gy. The 125I seeds were implanted intraoperatively or postoperatively. The regular follow-up was required. The Kaplan-Meier method was used to assess the tumor control rate and the patients' survival rates. Meanwhile, the Cox regression analysis was used to find out the prognostic factors.@*RESULTS@#Local control rates at the end of 3 and 5 years were as follows: T1-T2, 92.2% and 82.0%; T3-T4, 82.6% and 82.6%; and overall, 90.0% and 78.8%. The disease-free survival rates were 74.9% and 54.3%, respectively. The overall survival rates for all the patients were 86.0% and 79.6%, respectively at the end of 3 and 5 years and were 91.3% and 91.3% for T1-T2 patients vs. 73.9% and 59.7% for T3-T4 patients. Distant metastasis-free survival rates at the end of 3 and 5 years were 84.4% and 76.7%, respectively. The distant metastasis-free survival rates at the end of 3 and 5 years were 83.4% and 79.6% with T1-T2 lesion compared with 86.0% and 67.8% with T3-T4 lesion. According to the COX univariate analysis and multivariate analysis, the risk of local recurrence would be raised by the age. Tumor stage and tumor site were the prognostic factors of the overall survival rates.@*CONCLUSION@#125I brachytherapy conducted as an adjuvant therapy postoperatively of ACC of oral and maxillofacial region can acquire satisfactory localregional control, distant metastasis-free survival, disease-free survival and overall survival. Tumors are prone to recur on the older patients. Patients having advanced tumor stage or tumor located in the nasal cavity or sinuses will suffer lower survival rates.

Simulation of dose distribution in bone medium of 125I photon emitting source with Monte Carlo method / 北京大学学报(医学版)

OBJECTIVE@#To present a theoretical analysis of how the presence of bone in interstitial brachytherapy affects dose rate distributions with MCNP4C Monte Carlo code and to prepare for the next clinical study on the dose distribution of interstitial brachytherapy in head and neck neoplasm.@*METHODS@#Type 6711,125I brachytherapy source was simulated with MCNP4C Monte Carlo code whose cross section library was DLC-200. The dose distribution along the transverse axis in water and dose constant were compared with the American Association of Physicists in Medicine (AAPM) TG43UI update dosimetry formalism and current literature. The validated computer code was then applied to simple homogeneous bone tissue model to determine the affected different bone tissue had on dose distribution from 125I interstitial implant.@*RESULTS@#125I brachytherapy source simulated with MCNP4C Monte Carlo code met the requirements of TG43UI report. Dose rate constant, 0.977 78 cGy/(h×U), was in agreement within 1.32% compared with the recommended value of TG43UI. There was a good agreement between TG43UI about the dosimetric parameters at distances of 1 to 10 cm along the transverse axis of the 125I source established by MCNP4C and current published data. And the dose distribution of 125I photon emitting source in different bone tissue was calculated. Dose-deposition capacity of photons was in decreasing order: cortical bone, spongy bone, cartilage, yellow bone marrow, red bone marrow in the same medium depth. Photons deposited significantly in traversal axis among the phantom material of cortical bone and sponge bone relevant to the dose to water. In the medium depth of 0.01 cm, 0.1 cm, and 1 cm, the dose in the cortical bone was 12.90 times, 9.72 times, and 0.30 times of water respectively.@*CONCLUSION@#This study build a 125I source model with MCNP4C Monte Carlo code, which is validated, and could be used in subsequent study. Dose distribution of photons in different bone medium is not the same as water, and its main energy deposits in bone medium surface, so we should consider the effect of bone medium when we design the target area adjacent to the bone tissue in 125I sources implantation plan.

Design and Clinical Application of Blood Purification Management System / 医学信息学杂志

The paper introduces system requirements,feasibility,building target,functions and standard procedure of the blood pur ification management system,which is researched and developed independently,explains its development and operation environment,commonly used modules,permission management and other aspects.The system is able to enhance working efficiency and management quality and reduce medical risks.

Efficacy of dual antiplatelet therapy combined with Naoxintong capsules see text following coronary microembolization induced by homologous microthrombi in rats / 中国结合医学杂志

<p><b>OBJECTIVE</b>To evaluate the effificacy of dual antiplatelet therapy combined with Naoxintong Capsule ([see text], NXTC) in a rat model of coronary microembolization (CME).</p><p><b>METHODS</b>A total of 95 rats were randomly divided into 6 groups: control, sham-operation, CME model, NXTC, dual antiplatelet (clopidogrel and aspirin) intervention (DA), and NXTC combined with DA (NDA) groups. The complete data in 69 rats were obtained. The number of CME, myocardial apoptosis rate, bleeding time, clotting time, and adensosine diphosphate (ADP)-induced platelet aggregation were assessed.</p><p><b>RESULTS</b>Compared with the CME group, the number of CME and myocardial apoptosis rates were signifificantly decreased in the NXTC, DA, and NDA groups (P <0.01). Compared with other intervention groups, the number of CME and myocardial apoptosis rates were the least in the NDA group (P <0.01), and the incidence of surgical bleeding was the highest in the DA group (P <0.01). Compared with the CME group, ADP-induced maximum platelet aggregation rate was significantly inhibited in the NXTC, DA, and NDA groups (P <0.01), both bleeding time and clotting time were signifificantly increased in the NXTC, DA, and NDA groups (P <0.01), while the above parameters were the highest in the DA group (P <0.05).</p><p><b>CONCLUSION</b>The combination therapy of NXTC and DA enhanced the anti-CME effect of either therapy alone and reduced the risk of the DA therapy-associated bleeding, demonstrating an improved benefifit/ risk ratio in the rat model of CME.</p>

Test of dose distribution of palatal denture applicator containing (125)I seeds / 中华口腔医学杂志

<p><b>OBJECTIVE</b>to measure the dose distribution (tissue absorbed dose) of palatal denture applicator containing (125)I.</p><p><b>METHODS</b>simulated model of head and neck was used to wear the palatal dental applicator containing (125)I for the postoperative brachytherapy of a simulated tumor at a diameter of 2 cm in the palate. The denture contained 11 (125)I seed with radioactivity of 29.6 MBq per seed. The prescribed dose (edge matched dose) was 80 Gy. The absorbed dose in the simulated target and its adjacent area was measured by thermoluminescence dosimeters.</p><p><b>RESULTS</b>the dose in the target area reached the value of treatment needs, and the dose absorbed by tissue around the target was lower except tongue.</p><p><b>CONCLUSIONS</b>using palatal denture applicator containing (125)I for postoperative brachytherapy of malignant tumors of palate can get satisfied dose distribution, but the tongue needs to be protected.</p>

The technic and dose evaluation of postoperative (125)I radioactive seeds brachytherapy for malignant tumors of maxilla / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the technique and effect of treating malignant tumors of maxilla with postoperative brachytherapy by digital model obturators containing (125)I radioactive seeds.</p><p><b>METHODS</b>Four patients with adnoid cystic carcinomas and two with squamous cell carcinomas in the maxilla were included in this study. All patients accepted (125)I seeds implantation after operation, and wore digital model obturators containing (125)I seeds. The prescription dose was 80 Gy. All patients were followed up every 2 months after treatment.</p><p><b>RESULTS</b>The total number of seeds used in these six patients was 189, and 105 seeds were contained in obturators. Radioactive seeds and doses distributed very well as designed in all patients. No tumors recurred after follow up of 12-16 months and there were no severe complications.</p><p><b>CONCLUSIONS</b>It's an effective way to treat malignant tumors with positive excision margin in the maxilla by brachytherapy using digital model obturators which contain (125)I radioactive seeds.</p>

CT guided interstitial 125I seed implantation treatment for head and neck malignant tumor / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To detect the methods and experience of CT guided interstitial mj seed implantation treatment for head and neck malignant tumor.</p><p><b>METHODS</b>5 patients with head and neck malignant tumor were treated by interstitial 125I seed implantation with CT guide, 3 of them were treated with operation and adjunct seeds implantation, the other two patients were implanted seed only. According the treatment plan, register needles were punctured firstly, based on CT scan, these needle' s position and depth were adjusted, then the seeds were implanted into the target area. Another CT scan was done for the dose verification.</p><p><b>RESULTS</b>The procedure of 125I seeds implantation of five patients was successful. 5 of twelve register needles were adjusted. The dose distribution of the instant verification was the same as that of treatment plan.</p><p><b>CONCLUSION</b>The CT guided seeds implantation of the head neck malignant tumors can practice the treatment plan accurately, and the dose verification can be done instantly.</p>