ABSTRACT
Danshen Chuanxiongqin Injection is a commonly used medicine in cerebral infarction and coronary heart disease,which is recommended by many disease guidelines/consensus for cardiovascular and cerebrovascular diseases. However,there are irrational drug using in clinic,which affects the efficacy and brings safety risks. Based on clinical research evidence and expert experience,recommendations/consensus suggestion are determined through the nominal group method. The expert consensus recommends the indications,intervention time for treatment,route of drug administration,usage and dosage,course of treatment and introduces the safety in clinical application,which could provide reference for clinical use of Danshen Chuanxiongqin Injection.
Subject(s)
Humans , Cerebral Infarction , Drug Therapy , Consensus , Drugs, Chinese Herbal , Therapeutic Uses , InjectionsABSTRACT
<p><b>OBJECTIVE</b>To study the effects of yiqi huoxue recipe and Coxsackie B virus type 3 (CVB3) on the expression of ribosomal protein S20 in rat cardiac myocytes, to explore the pathogenesis of myocarditis induced by CVB3 and the mechanism of yiqi huoxue recipe on gene level, and to further investigate whether yiqi huoxue recipe is an effective prescription for CVB3 myocarditis.</p><p><b>METHODS</b>A modified suppression subtractive hybridization (SSH) was used to isolate differentially expressed genes between the CVB3 infection group and the treatment group with yiqi huoxue recipe. The results were further verified by fluorescence RT-PCR.</p><p><b>RESULTS</b>The results of SSH showed that the gene expression of ribosomal protein S20 in the treatment group was higher than that in the CVB3 infection group (P<0.05), which agreed with the results of fluorescent RT-PCR.</p><p><b>CONCLUSION</b>Down-regulation of ribosomal protein S20 mRNA expression might be one of the mechanisms in CVB3 myocarditis, and yiqi huoxue recipe could achieve the treatment of viral myocarditis by regulating the expression of ribosomal protein S20.</p>
Subject(s)
Animals , Rats , Coxsackievirus Infections , Drug Therapy , Virology , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Enterovirus B, Human , Metabolism , Gene Expression Regulation , Myocytes, Cardiac , Metabolism , Virology , Nucleic Acid Hybridization , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Ribosomal Proteins , Genetics , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of Chinese herbal compound for supplementing qi and activating blood circulation (CHC) on the gap junctional intercellular communication (GJIC) function of myocardial cells in patients with Coxsackie virus B 3 (CVB3) viral myocarditis.</p><p><b>METHODS</b>Expressions of actin and connexin43 (Cx43) in myocardial cells of patients arranged in three groups (the normal control group, the viral infected group and the CHC treated group) were detected by immunohistochemical method; the fluorescence photobleaching recovery rate of cells was detected by laser scanning confocal microscope.</p><p><b>RESULTS</b>As compared with the viral infected group, the expressions of actin and Cx43 were increased and the GJIC function was improved in the CHC treated group.</p><p><b>CONCLUSION</b>CHC could antagonize viral injury on skeleton protein, and repair the structure of gap junction channel to improve the GJIC function of myocardial cells after being attacked by CVB3.</p>