Studies of sepia influence on NK cells activity and effect on in- hibition tumor in mice / 中国海洋药物

Study on sepia effect on NK cells killing activity and inhibit tumor growth in the load tumor mice, normal and load tumor mice were poured into sepia through mouth, examining on activity of NK cells and lasted time of it . Through pathological diagnosis of tumor tissues and NK cells killing activity for load tumor mice judge effect on sepia . The results showed that the sepia can increase spleen cells function of natural killer cells and it can last 7 days , and prompt tumor tissues large area necrosis and inflammatory cells infiltration. To compared them with control groups, it is obvious that the sepia can inhibit tumor growth and to increase activity of NK cells.

Surgical treatment and perioperative management of carotid body tumors / 中国普通外科杂志

Objective To explore the surgical treatment and perioperative management of carotid body tumors.Methods Fifteen patients with carotid body tumor were operated upon after appropriate perioperative management.Results Among the 15 cases,6 underwent simple tumor excision,7 had co-resection of carotid body tumor and carotid artery,and 2 cases had arterial anastomosis of common carotid artery and internal carotid artery after co-resection of carotid body tumor.There was no mortality after operation in this series.All patients were followed up from 1 year to 3 years (average,15.3months).Operative incisions healed by primary intention.The pressure symptoms of carotid body tumor disappeared after operation,but two patients had temporary headache for 3months,and one patient died of cerebral infarction.Injury of hypoglossal nerve occurred in one patient but it recovered after treatment.There were no recurrences of carotid body tumor after operation.Conclusions Adequate perioperative management can improve the effect and safety of operation,and decrease operative complications of patients with carotid body tumor.

THE EXPERIMENTAL MORPHOLOGICAL OBSERVATION ON HAMSTER TRACHEA IN ORGAN CULTURE / 解剖学报

This article deals with the organ culture on hamster trachea under the normal condition. The result showed that the epithelium of trachea could be kept alive up to 8 th-week at most by means of rotatory method of incubation. The culture media are RPMI 1640 (Nissui) and Eagle MEM (Nissui). Each medium was with or without bovine serum added. The results of histological, ultrastructural, radioautographic observations exhibited that the tracheal epithelia of hamster before culture showed ciliated columnar form with cilia. With prolongation of culture time, the changes of tracheal epithelium took the following order, from ciliated columnar to simple columnar and to simple squamous form. These changes were observed in all 4 groups. In radioautography, there are labeling granules in nuclei of tracheal epithelial cells in all 4 groups at different culture time, indicating that the epithelial cells still maintained DNA synthetic activity. TEM observation has showed ultrastructure of tracheal epithelium remained unchanged within 4 weeks. The ciliated cells are well with intact cilia. The intercellular junction is the tight one. Occasionally in accordance with LM observation a few of ciliated cell lacked cilia and its nucleus flattened. In cytoplasm, the dilatation of cisternae of rER and the incresing amount of ribosomes and lysosomes could be seen under TEM. During 7-8 weeks only a part of epithelia of trachea was still kept well, most of them became single layer. The ciliated cells lacked cilia, and their nuclei were long and oval in shape. Some enlarged lysosomes and autophagic vacuoles could be seen in cytoplasm indicating cytoplasmic degeneration. The present study suggested that both Eagle MEM and RPMF 1640 show the same culture effect.