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ObjectiveTo explore the relationship of one-child attachment and parerttal attachment,as well as the effect of family income and parental education on children's attachment.Methods 350 children aged over 12 years from primary school grade 5 to high school grade 3 in Nanjing were selected in two classes each grade in cluster sampling method as research objects.These children and their parents were measured by General questionnaire,Experiences in Close Relationships Inventory( ECR ) and Adolescent Attachment Inventory.The data of the questionnaires were coded for statistical analysis-Pearson conrelation to analyze the relationship of one-child attachment and parental attachment and Analyze of variance to explore the influence of family income and parental education on children's attachment.ResultsThere was a significantly negative relation(r =-0.132,P=0.014)between one-child family negative dimension and mother avoidance dimension,and a significant correlation (r =0.131,P =0.015 ) between one-child family negative dimension and mother anxiety dimension.The interaction of family income and parental culture was significant in affinity attachment of one-child (F =3.641,4.052,P =0.006,0.003).ConclusionThis study finds that one-child is more attached to their mothers than their fathers.Family income and parental education affect the attachment of one-child.
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ObjectiveTo explore the characteristics of family dynamics in different developmental disorders.MethodsIn this study cross-sectional survey were used.Parents of 69 Autistic Disorder (AD) children,18 Asperger Syndrome (AS) children,51 Mental Retardation (MR) children and 69 Normal Group (NG) children were investigated by Self-rating Inventory of Systemic Family Dynamics to assess their family dynamic characteristics.SPSS18.0 package for statistics were used.ResultsCovariance analysis by controlling children's age showed:there were significant differences in both fathcrs'and mothers'family atmosphere,systemic logic and disease concept dimension among the tour groups ( Ffather =5.355,3.946,6.961,P＜ 0.05 ; Fmother =4.325,2.977,5.878,P ＜ 0.05 ).Pairwise comparison indicated that scores of both fathers'and mothers'family atmosphere,disease concept dimension in AD ( father ( 24.24 ± 5.89 ),( 10.60 ± 2.81 ) ; mother ( 24.90 ± 8.18 ),( 10.59 ± 3.07 ) ),AS ( father (24.67±7.07),(11.39 ±2.52); mother (26.83 ±6.24),(10.61 ±2.55)),MR(father (25.02 ±7.89 ),( 10.94 ± 2.94 ) ;mother( 24.02 ± 7.59 ),( 11.42 ± 3.54 ) ) groups were significant higher than that of NG (father (20.19 ± 5.75 ),( 8.74 ± 2.63 ) ; mother ( 20.87 ± 5.85 ),( 8.88 ± 2.64 ) ) (P ＜ 0.05 ).Scores of both fathers'and mothers'systemic logic dimension in MR group( father ( 24.26 ± 4.36 ),mother( 23.54 ± 4.58 ) ) were higher than that of NG(father (21.61 ±3.97),mother (21.03 ±3.66),P＜0.01).ConclusionIn families of AD,AS and MR children,the family atmosphere is more boring,parents are more likely to consider themselvesfull of victims.Parents of MR children are more prone to one or the other way of looking at problems.
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Objective To investigate the family dynamic characteristics of adolescents with emotional disorder.Methods 74 adolescents with emotional disorder from Child Mental Health Research Center of Nanjing Brain Hospital Affiliated to Nanjing Medical University were chosen and compared with 148 gender- and agematched controls from schools in Nanjing.Self-rating scale of systemic family dynamics(SSFD) was used to assess the characteristics of family dynamics,and general demographic information inventory was conducted for all subjects.Results Adolescents with emotional disorder showed higher scores in family atmosphere and systemic logic than control group ( ( 30.54 ± 8.28 ) vs (23.45 ± 7.40),( 17.14 ± 4.26 ) vs ( 15.43 -± 3.86 ),all P ＜ 0.01 ).Logistic regression analysis showed that high individuation ( OR =0.903,95％ CI:0.834 ～ 0.977 ) and high disease thinking( OR =0.853,95％ CI:0.750 ～ 0.970 ) were protective factors for emotional disorder.High family atmosphere ( OR =1.167,95％ CI:1.101 ～ 1.236) was risk factor for emotional disorder.Conclusion Adolescents with emotional disorder demonstrate boring and hostile family atmosphere and Either/or family systemic logic on the family dynamic characteristics.Family atmosphere,disease thinking and individuation are associated with emotional disorder.
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Objective To investigate the features of parental attachment in children with attention deficit hyperactivity disorder(ADHD).Methods Experience in Close Relationship Scale Revised(ECR-R),Relationship Questionnaire(RQ) and Homemade General Situation Table was conducted among 164 parents of ADHD and 328 parents of normal children.Results The attachment repretation in fathers of children with ADHD showed that autonomous(43.9％ ) was lower than those of the control group (52.4％),dismissing type (41.5％ ) was higher than the normal group (34.1％),preoccupied( 12.2％ ) was higher than those in the control group (8.0％),unresolved type (2.4％) was lower than those of the control group (5.5％),the difference was not statistically significant (P ＞ 0.05 ).The attachment repretation in mothers of children with ADHD showed that autonomous (40.2％) was lower than those of the control group (50.0％),dismissing type (30.5％) belowed the normal group (32.3％),preoccupied type ( 20.7％ ) higher than those in the control group ( 10.4％ ),unresolved type (8.5％) higher than those in the control group (7.3％),the difference was not statistically significant (P ＞0.05).Two groups had no statistical differences in paternal attachment anxiety,paternal attachment avoidance、maternal attachment anxiety and maternal attachment avoidance dimensions ( ( 3.44 + 0.97 ) vs ( 3.37 + 0.82 ),(3.70+0.57)vs(3.72 +0.57),(3.37+0.87) vs(3.36 +0.83),(3.74+0.68)vs(3.64±0.59),allP＞0.05).Conclusion There is no significant differences in the features of parental attachment between children with ADHD and normal children.
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Objective To discuss curative effects of the AO Less Invasive Stabilization System(LISS) in the treatment of complex fractures around the knee joint.Methods The internal fixation at distal femur or proximal tibia was performed in 15 cases of closed complex fractures at the metaphysic area near the knee joint by using the LISS(left-sided in 9 cases and right-sided in 6 cases) from April 2004 to June 2005. Results All the 15 cases were followed for 5~26 months(mean,13.2 months).The time to full weight bearing was 12~26 weeks,and the time to bone union under X-ray was 11~24 weeks.No delayed union or disunion was observed.There were 1 case of superficial infection and 2 cases of skin blister,all of which were cured with dressing changes.No deep infection,skin necrosis,or osteofascial compartment syndrome occurred.Functional evaluation according to the Johner-Wruhs system revealed "excellent" results in 11 cases,"good" results in 3,and "fair" in 1,the total satisfactory rate being 93.3%(14/15).Palpable shift and separation inside tibia had occurred in 1 case of tibial plateau fracture(type C3) 2 weeks after operation,and a steel bar was inserted to fix the tibial plateau in a second operation.Bone union was achieved in this case 20 weeks after operation,with "good" evaluation results.Genu varum(tibial plateau angle,82?) had occurred in 1 case of tibial plateau fracture(type C3) 8 weeks after operation,and bone union was noted 20 weeks after operation,with "fair" evaluation results.Conclusions The LISS is indicated for fractures at distal femur or proximal tibia,or osteoporotic fractures of distal femoral shaft.For type C3 complex tibial plateau fracture,fixation with a steel bar on the entocondyle should be employed,rather than relying on the LISS.
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Objective To explore feasibility and clinic results of the MIPPO (minimally invasive percutaneous plate osteosynthesis) technique in treatment of humeral fractures. Methods 14 patients with humeral fracture underwent the MIPPO operation in our department. 7 of them were injured in a traffic accident, 4 in sports and 3 in daily life. There were 11 males and 3 females, with their ages ranging from 16 to 72 years. Of the 14 cases, 9 were proximal ones and 5 mid- distal ones, 3 were multiple injuries and 1 pathological fracture. Results The average intra- operative time was 80 minutes (ranging from 55 to 130 minutes). No patient received blood transfusion. The intra- operative blood loss was 100 to 200 mL. 14 cases were followed up for 8 weeks to 14 months. Their cuts healed at the primary stage. Their shoulder and elbow functions were fine. The fracture segments got satisfactory reduction with good apposition and alignment radiologically. The callus appeared 4 to 8 weeks postoperatively. Conclusion MIPPO is a safe and effective treatment for the humeral fracture with the benefits of less invasion, fewer complications and higher union rate.
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Objective To measure serum thrombopoietin(TPO)levels in thrombocytopenic conditions of aplastic anemia(AA)and idiopathic thrombocytopenic purpura(ITP)and explore the clinical significance of the changes of TPO levels.Methods Serum TPO concentration was determined in AA(n=13),ITP(n=20)and normal controls(n=17)by using an enzyme linked immunosorbent assay(ELISA).Results Serum TPO level in AA patients was much higher than that in normal controls[(683^48?414^73)ng/L vs (99^41?73^84)ng/L,P0 05].Furthermore,there was an inverse correlation between TPO concentration and platelet count in AA patients(r=-0^71,P
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AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38-and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers.
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Transcription factor GATA-2 is a member of GATA family which binds a common DNA sequence motif [T/A (GATA) A/G] through an evolutionarily conserved zinc finger domain. An essential role for GATA-2 in the development of hematopoietic cells has recently been shown in gene knockout experiments in mice. GATA-2 gene disruption makes reduction in all hematopoietic precursors, whereas enforced expression of GATA-2 blocks normal hematopoiesis. GATA-2 exerts regulation in early embryo and cooperates with other GATA transcription factors in proliferation and differentiation of myeloid, erythroid, megakaryocytic and mast cell lineages. GATA-2 mRNA and protein could be detected in human myelogenous leukemia cell lines and in most patients with primary leukemias. Some data revealed that GATA-2 could transactivate the Cas-Br-E and graffi retroviruses, which can induce myelogenous leukemia in mice. However, the role of GATA-2 in pathogenesis of leukemia is still not well understood.
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AIM: To establish a arsenic trioxide (As 2O 3 )-resistant leukemic cell line to explore the mechanism of resistance to As 2O 3, and the relationship between the resistant cell line and the multidrug resistance was also investigated. METHODS: The arsenic trioxide (As 2O 3 )-resistant leukemic cell line was established by exposing the cells to the increasing concentration of As 2O 3. MTT assay was used to detect the cytotoxicity. Cell cycle was detected by PI assay. Flow Cytometry was used to detect the P-glycoprotein on the surface of the cells, the intracellular concentration of DNR, and the immuetype of the cells. RESULTS: The cell doublings time and the cell cycle of the arsenic trioxide (As 2O 3 )-resistant leukemic cell line, K562/AS2, is similar to that of K562. The relative resistant fold of K562/AS2 to As 2O 3, DNR, VP16 and Ara-C was 7.4, 2.9, 3.8 and 1.1, respectively. The relative resistant fold of multidrug resistant cell line, K562/ A02, to As 2O 3, DNR, VP16 and Ara-C was 0.8?94?2.5 and 0.9, respectively. The fluorescence of the P-glycoprotein on the surface or of the DNR inside the cells detected was not significantly different between the K562 and the K562/AS2 cell lines. CONCLUSIONS: A cell line, K562/AS2, resistant to clinical achieving level (2 ?mol/L) of As 2O 3 has been established. The relative resistant fold of K562/ AS2 to As 2O 3 is about 7.4 fold to the parent K562 line sensitive to As 2O 3. Partial resistance of K562/AS2 to DNR and VP16 is observed , the mechanism of which is unrelated to the P-gp, the expression product of multidrug resistance gene 1 (mdr1).
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AIM:To explore the changes of the subsets and HLA-DR expression of dendritic cells and their concerning cytokine levels in peripheral blood of patients with breast cancer.METHODS:The subsets of the precussors of dendritic cells(pDC)in the peripheral blood of 57 cases of patients with breast cancer before operation and a week or six months after operation and 20 cases of healthy controls were analyzed by four-color FCM.The levels of IL-12p40,IL-10,IFN-? and IL-4 in the plasmas were tested by ELISA.RESULTS:Among 57 cases of patients with breast cancer,2 cases in Ⅲ phase and 4 cases in Ⅳphase expressed deficiency of pDC,the ratios of pDC1/pDC2 in the other cases inⅠ,Ⅱ,Ⅲ,Ⅳ phase were respectively 1.62?0.59,1.41?0.63,0.91?0.32,0.81?0.29 before operation,which were markedly lower than those in controls(1.94?0.44).The ratios of pDC1/pDC2 in the cases inⅠ,Ⅱ,Ⅲ phase were 1.71?0.47,1.52?0.54,1.04?0.36 a week after operation,which were the same as those in pre-operation,but markedly lower than those in controls.The ratios of pDC1/pDC2 in the cases inⅠ,Ⅱ,Ⅲ phase were 1.92?0.72,1.63?0.65,1.28?0.34 six months after operation,which were markedly higher than those in pre-operation,meanwhile,to compare with controls,those were still lower for patients in Ⅱ,Ⅲ phase except in Ⅰphase.No difference between patients and controls in the expression of HLA-DR of pDCs and the levels of IL-12p40,IL-10,IFN-?,IL-4 in plasmas and the ratios of IL-12p40/IL-10,IFN-?/IL-4 was observed.CONCLUSION:The ratios of pDC1/pDC2 in peripheral blood of patients with breast cancer inⅠ-Ⅳ phase are decreased.Parts of patients in Ⅲ,Ⅳ phase are deficiency of pDCs.HLA-DR expression of DCs and the ability of DCs which secret the concerning cytokines do not change as pDC subsets change.pDC subsets improve markedly inⅡ,Ⅲ phase patients and recover to the normal level inⅠphase patients after operation.
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AIM: To investigate the effect of CD_8+ cells from aplastic anemia (AA) patients and its histamine type II (H_2) receptors on the growth of normal CFU-Mk. METHODS: The effects of CD_8+ cells and/or cimetidine, on normal human CFU-Mk growth were studied by using CFU-Mk assay. RESULTS: The CD_8+ cells from the perpheral blood of AA patients significantly suppressed the growth of normal allogeneic CFU-Mk. This inhibitory effect was blocked by cimetidine at concentration of 1.0?10~-5 mol/L. 1.0?10~-5 mol/L cimitidine alone didn't inhibit the growth of normal CFU-Mk. CONCLUSION: H_2 receptor antagonist cimitidine abolishes the suppressive effect of AA patients CD_8+ cells on the growth of normal CFU-Mk.
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AIM:To study the effects of thrombopoietin (TPO) and other hematopoietic cytokines on platelet activation. METHODS: Using fluorescent-labeled monoclonal antibodies and flow cytometry.RESULTS: The per- centage range of platelets activated by 120 ng/mL TPO was 8. 89% -39. 92%,mean value was 17.43%; However, there were no effects of TPO 40 ng/mL and GM - CSF 100 ng/mL and IL- 3 100 ng/mL on platelet activation. CONCLUSION: Higher concentration of TPO directly stimulated platelet activation.
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0.2mM Cytidine 3', 5'-monophosphate (3', 5'-cCMP) inhibits not only the IL-2 production of human peripheral blood mononuclear cells (PBMC) but also the expression of IL-2 receptors. The inhibitory rate on expression of IL-2 receptors is 28.9+1.9%. The proliferation of CTLL-2 cells can be stimulated by 3', 5'-cCMP in concentration from 0.00625mM to 0.2mM when the presence of IL-2 is sufficient. The stimulative effect in low concentration of 3', 5'-cCMP is more obvious than that in high concentration.
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The effect of plasma exchange (PE) in vivo was studied. The significant reductions of Cl~-, albumin, globulin, IgG, CIC and fibrinogen were observed after plasmapheresis, but Na~+, K~+, Ca~(++), Mg~(++), Cr, BUN and C3 hadn't significant changes, while the percentage of T suppressor cells increased after PE. The possibility of thrombosis should be awared, because hypercoagulation might be induced by mechanical depletion of antithrombin Ⅲ.
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The T-lymphoblastic JM cell line was used to study the influence of the 3′, 5′-cCMP on cell growth. S-Phase parasynchronization was induced by incubation with 0.2mM thymidine for 20 hrs. M-phase parasynchronization was induced by a two step method: Incubation with thymidine was performed first as described above. 11 hours after cell release, colcemide 0.1?g/ml was added to act for 6 hrs. Incubatiou with 3′,5′-cCMP was performed at the time of release. Flow cytometry was performed by an Ortho Cytofluograph System 30.3′, 5′-cCMP can inhibit cell growth during the period from S-phase to G_2/M-phase aud from G_1-phase to S-pase as well. In contrast, 5-CMP, 2′,3′-cCMP and 5-dCMP have no inhibiting effect on cell growth, but can stimulate cell growth in S-phase. Inhibiting effect of 3′,5′-cCMP on cell growth was also present in asynchronous cell cultures.